PMID- 10500206
OWN - NLM
STAT- MEDLINE
DCOM- 19991021
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 20
DP  - 1999 Sep 28
TI  - Mice without phosphatidylcholine transfer protein have no defects in the
      secretion of phosphatidylcholine into bile or into lung airspaces.
PG  - 11501-6
AB  - Phosphatidylcholine transfer protein (Pc-tp) is a highly specific carrier of
      phosphatidylcholine (PC) without known function. Proposed functions include the
      supply of PC required for secretion into bile or lung air space (surfactant) and 
      the facilitation of enzymatic reactions involving PC synthesis or breakdown. To
      test these functions, we generated knock-out mice unable to make Pc-tp.
      Remarkably, these mice are normal and have no defect in any of the postulated
      Pc-tp functions analyzed. The lipid content and composition of the bile, as well 
      as lung surfactant secretion and composition, of Pc-tp (-/-) mice, is normal. The
      lack of a Pc-tp contribution to biliary lipid secretion is in agreement with our 
      finding that Pc-tp is down-regulated in adult mouse liver: whereas Pc-tp is
      abundant in the liver of mouse pups, Pc-tp levels decrease > 10-fold around 2 wk 
      after birth, when bile formation starts. In adult mice, Pc-tp levels are high
      only in epididymis, testis, kidney, and bone marrow-derived mast cells. Absence
      of Pc-tp in bone marrow-derived mast cells does not affect their lipid
      composition or PC synthesis and degradation. We discuss how PC might reach the
      canalicular membrane of the hepatocyte for secretion into the bile, if not by
      Pc-tp.
FAU - van Helvoort, A
AU  - van Helvoort A
AD  - Division of Molecular Biology, Center of Biomedical Genetics, The Netherlands
      Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands.
FAU - de Brouwer, A
AU  - de Brouwer A
FAU - Ottenhoff, R
AU  - Ottenhoff R
FAU - Brouwers, J F
AU  - Brouwers JF
FAU - Wijnholds, J
AU  - Wijnholds J
FAU - Beijnen, J H
AU  - Beijnen JH
FAU - Rijneveld, A
AU  - Rijneveld A
FAU - van der Poll, T
AU  - van der Poll T
FAU - van der Valk, M A
AU  - van der Valk MA
FAU - Majoor, D
AU  - Majoor D
FAU - Voorhout, W
AU  - Voorhout W
FAU - Wirtz, K W
AU  - Wirtz KW
FAU - Elferink, R P
AU  - Elferink RP
FAU - Borst, P
AU  - Borst P
LA  - eng
SI  - GENBANK/AF151638
SI  - GENBANK/AF151639
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Androgen-Binding Protein)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Leukotrienes)
RN  - 0 (PEBP1 protein, human)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Phosphatidylethanolamine Binding Protein)
RN  - 0 (Phospholipid Transfer Proteins)
RN  - 0 (Pulmonary Surfactants)
SB  - IM
MH  - Amino Acid Sequence
MH  - *Androgen-Binding Protein
MH  - Animals
MH  - Bile/*metabolism
MH  - Carrier Proteins/genetics/*physiology
MH  - Cells, Cultured
MH  - DNA, Complementary/analysis
MH  - Humans
MH  - Leukotrienes/biosynthesis
MH  - Lung/*metabolism
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phosphatidylcholines/*metabolism
MH  - Phosphatidylethanolamine Binding Protein
MH  - Phospholipid Transfer Proteins
MH  - Pulmonary Surfactants/biosynthesis
PMC - PMC18063
EDAT- 1999/09/29 00:00
MHDA- 1999/09/29 00:01
CRDT- 1999/09/29 00:00
PHST- 1999/09/29 00:00 [pubmed]
PHST- 1999/09/29 00:01 [medline]
PHST- 1999/09/29 00:00 [entrez]
AID - 10.1073/pnas.96.20.11501 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11501-6. doi:
      10.1073/pnas.96.20.11501.