PMID- 10500199
OWN - NLM
STAT- MEDLINE
DCOM- 19991021
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 20
DP  - 1999 Sep 28
TI  - Oncogenic homeodomain transcription factor E2A-Pbx1 activates a novel WNT gene in
      pre-B acute lymphoblastoid leukemia.
PG  - 11464-9
AB  - A large fraction of pediatric pre-B acute lymphoblastoid leukemias (ALL)
      consistently contain a t(1;19) chromosomal translocation. The t(1;19)
      translocation results in the production of a chimeric transcription factor
      containing the N-terminal transactivation domain of E2A fused to the C-terminal
      DNA-binding homeodomain of Pbx1. Here, we show that the E2A-Pbx1 fusion protein
      activates the expression of a novel WNT gene, WNT-16. WNT-16 normally is
      expressed in peripheral lymphoid organs such as spleen, appendix, and lymph
      nodes, but not in bone marrow. In contrast, high levels of WNT-16 transcripts are
      present in bone marrow and cell lines derived from pre-B ALL patients carrying
      the E2A-Pbx1 hybrid gene. Inhibition of E2A-Pbx1 expression leads to a
      significant decrease in WNT-16 mRNA levels, suggesting that WNT-16 is a
      downstream target of E2A-Pbx1. Three putative WNT receptors, FZ-2, FZ-3, and
      FZ-5, are expressed in cells of the B lineage, including pre-B ALL cells
      aberrantly expressing WNT-16. We propose that a WNT-16-mediated autocrine growth 
      mechanism contributes to the development of t(1;19) pre-B ALL.
FAU - McWhirter, J R
AU  - McWhirter JR
AD  - Department of Biology, 0366, University of California at San Diego, La Jolla, CA 
      92093, USA.
FAU - Neuteboom, S T
AU  - Neuteboom ST
FAU - Wancewicz, E V
AU  - Wancewicz EV
FAU - Monia, B P
AU  - Monia BP
FAU - Downing, J R
AU  - Downing JR
FAU - Murre, C
AU  - Murre C
LA  - eng
SI  - GENBANK/AF169963
SI  - GENBANK/AF169964
SI  - GENBANK/AF172064
SI  - GENBANK/AH008358
GR  - P01 CA071907/CA/NCI NIH HHS/United States
GR  - P01 CA71907-03/CA/NCI NIH HHS/United States
GR  - R01 CA65797 05/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Wnt Proteins)
RN  - 0 (Zebrafish Proteins)
RN  - 146150-85-8 (E2A-Pbx1 fusion protein)
SB  - IM
MH  - Amino Acid Sequence
MH  - Gene Expression Regulation
MH  - Homeodomain Proteins/*physiology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Oncogene Proteins, Fusion/*physiology
MH  - Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - Proto-Oncogene Proteins/*genetics
MH  - Transcription Factors/*physiology
MH  - Tumor Cells, Cultured
MH  - Wnt Proteins
MH  - *Zebrafish Proteins
PMC - PMC18056
EDAT- 1999/09/29 00:00
MHDA- 1999/09/29 00:01
CRDT- 1999/09/29 00:00
PHST- 1999/09/29 00:00 [pubmed]
PHST- 1999/09/29 00:01 [medline]
PHST- 1999/09/29 00:00 [entrez]
AID - 10.1073/pnas.96.20.11464 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11464-9. doi:
      10.1073/pnas.96.20.11464.