PMID- 10500182
OWN - NLM
STAT- MEDLINE
DCOM- 19991021
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 20
DP  - 1999 Sep 28
TI  - RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination.
PG  - 11364-9
AB  - A RING finger-containing protein (AO7) that binds ubiquitin-conjugating enzymes
      (E2s) and is a substrate for E2-dependent ubiquitination was identified.
      Mutations of cation-coordinating residues within AO7's RING finger abolished
      ubiquitination, as did chelation of zinc. Several otherwise-unrelated RING finger
      proteins, including BRCA1, Siah-1, TRC8, NF-X1, kf-1, and Praja1, were assessed
      for their ability to facilitate E2-dependent ubiquitination. In all cases,
      ubiquitination was observed. The RING fingers were implicated directly in this
      activity through mutations of metal-coordinating residues or chelation of zinc.
      These findings suggest that a large number of RING finger-containing proteins,
      with otherwise diverse structures and functions, may play previously
      unappreciated roles in modulating protein levels via ubiquitination.
FAU - Lorick, K L
AU  - Lorick KL
AD  - Laboratory of Immune Cell Biology, Division of Basic Sciences, National Cancer
      Institute, Building 10, Room 1B34, National Institutes of Health, 9000 Rockville 
      Pike, Bethesda, MD 20892-1152, USA.
FAU - Jensen, J P
AU  - Jensen JP
FAU - Fang, S
AU  - Fang S
FAU - Ong, A M
AU  - Ong AM
FAU - Hatakeyama, S
AU  - Hatakeyama S
FAU - Weissman, A M
AU  - Weissman AM
LA  - eng
SI  - GENBANK/AF171060
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Ubiquitins)
RN  - EC 2.3.2.23 (UBE2D2 protein, human)
RN  - EC 2.3.2.23 (Ubiquitin-Conjugating Enzymes)
RN  - EC 6.- (Ligases)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Amino Acid Sequence
MH  - Ligases/genetics/*physiology
MH  - Molecular Sequence Data
MH  - *Ubiquitin-Conjugating Enzymes
MH  - Ubiquitins/*metabolism
MH  - Zinc/pharmacology
MH  - *Zinc Fingers
PMC - PMC18039
EDAT- 1999/09/29 00:00
MHDA- 1999/09/29 00:01
CRDT- 1999/09/29 00:00
PHST- 1999/09/29 00:00 [pubmed]
PHST- 1999/09/29 00:01 [medline]
PHST- 1999/09/29 00:00 [entrez]
AID - 10.1073/pnas.96.20.11364 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11364-9. doi:
      10.1073/pnas.96.20.11364.