PMID- 10500175
OWN - NLM
STAT- MEDLINE
DCOM- 19991021
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 20
DP  - 1999 Sep 28
TI  - Tubulointerstitial nephritis antigen: an extracellular matrix protein that
      selectively regulates tubulogenesis vs. glomerulogenesis during mammalian renal
      development.
PG  - 11323-8
AB  - Tubulointerstitial nephritis antigen (TIN-ag) is an extracellular matrix protein 
      and is expressed in the renal tubular basement membranes. Its role in metanephric
      development was investigated. TIN-ag cDNA, isolated from the newborn mouse
      library, had an ORF of 1,425 nucleotides, a putative signal sequence, and an
      ATP/GTP-binding site. The translated sequence had approximately 80% identity with
      rabbit TIN-ag. Among various tissues, TIN-ag mRNA was primarily expressed in the 
      newborn kidney. In the embryonic metanephros, TIN-ag expression was confined to
      the distal convolution or pole of the S-shaped body, the segment of the nascent
      nephron that is the progenitor of renal tubules. Treatment with TIN-ag antisense 
      oligodeoxynucleotide induced dysmorphogenesis of the embryonic metanephroi,
      malformation of the S-shaped body, and a decrease in the tubular population,
      whereas the glomeruli were unaffected. Treatment also led to a decrease of TIN-Ag
      mRNA, de novo synthesis of TIN-ag protein, and its antibody reactivity. The mRNA 
      expression of glomerular epithelial protein 1 (a marker for renal podocytes),
      anti-heparan-sulfate-proteoglycan antibody reactivity, and wheat germ agglutinin 
      lectin staining of the metanephros were unaffected. The anti-TIN-ag antibody
      treatment also caused deformation of the S-shaped body and a reduction in the
      tubular population, whereas the glomeruli were unchanged. The data suggest that
      the TIN-ag, unlike other basement membrane proteins, selectively regulates
      tubulogenesis, whereas glomerulogenesis is largely unaffected.
FAU - Kanwar, Y S
AU  - Kanwar YS
AD  - Department of Pathology, Northwestern University Medical School, Chicago, IL
      60611, USA. y-kanwar@nwu.edu
FAU - Kumar, A
AU  - Kumar A
FAU - Yang, Q
AU  - Yang Q
FAU - Tian, Y
AU  - Tian Y
FAU - Wada, J
AU  - Wada J
FAU - Kashihara, N
AU  - Kashihara N
FAU - Wallner, E I
AU  - Wallner EI
LA  - eng
SI  - GENBANK/AF153366
GR  - R01 DK028492/DK/NIDDK NIH HHS/United States
GR  - R37 DK028492/DK/NIDDK NIH HHS/United States
GR  - DK28492/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DNA, Complementary)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (RNA, Messenger)
RN  - 0 (Telomere-Binding Proteins)
RN  - 0 (Tinag protein, mouse)
RN  - 0 (Tinf2 protein, mouse)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
RN  - EC 3.1.3.48 (Ptpro protein, mouse)
RN  - EC 3.1.3.48 (Receptor-Like Protein Tyrosine Phosphatases, Class 3)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Adhesion Molecules/genetics/immunology/*physiology
MH  - DNA, Complementary/analysis
MH  - Extracellular Matrix Proteins/genetics/immunology/*physiology
MH  - Kidney Glomerulus/*embryology
MH  - Kidney Tubules/*embryology
MH  - Membrane Glycoproteins/genetics/immunology/*physiology
MH  - Membrane Proteins/genetics
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Molecular Sequence Data
MH  - Nephritis, Interstitial/*immunology
MH  - Oligonucleotides, Antisense/pharmacology
MH  - Protein Tyrosine Phosphatases/genetics
MH  - RNA, Messenger/analysis
MH  - Rabbits
MH  - Receptor-Like Protein Tyrosine Phosphatases, Class 3
MH  - *Telomere-Binding Proteins
PMC - PMC18032
EDAT- 1999/09/29 00:00
MHDA- 1999/09/29 00:01
CRDT- 1999/09/29 00:00
PHST- 1999/09/29 00:00 [pubmed]
PHST- 1999/09/29 00:01 [medline]
PHST- 1999/09/29 00:00 [entrez]
AID - 10.1073/pnas.96.20.11323 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11323-8. doi:
      10.1073/pnas.96.20.11323.