PMID- 10500174
OWN - NLM
STAT- MEDLINE
DCOM- 19991021
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 20
DP  - 1999 Sep 28
TI  - Expression of the CDH1-associated form of the anaphase-promoting complex in
      postmitotic neurons.
PG  - 11317-22
AB  - The anaphase-promoting complex/cyclosome (APC) is a tightly cell cycle-regulated 
      ubiquitin-protein ligase that targets cyclin B and other destruction
      box-containing proteins for proteolysis at the end of mitosis and in G1. Recent
      work has shown that activation of the APC in mitosis depends on CDC20, whereas
      APC is maintained active in G1 via association with the CDC20-related protein
      CDH1. Here we show that the mitotic activator CDC20 is the only component of the 
      APC ubiquitination pathway whose expression is restricted to proliferating cells,
      whereas the APC and CDH1 are also expressed in several mammalian tissues that
      predominantly contain differentiated cells, such as adult brain.
      Immunocytochemical analyses of cultured rat hippocampal neurons and of mouse and 
      human brain sections indicate that the APC and CDH1 are ubiquitously expressed in
      the nuclei of postmitotic terminally differentiated neurons. The APC purified
      from brain contains all core subunits known from proliferating cells and is
      tightly associated with CDH1. Purified brain APC(CDH1) has a high cyclin B
      ubiquitination activity that depends less on the destruction box than on the
      activity of mitotic APC(CDC20). On the basis of these results, we propose that
      the functions of APC(CDH1) are not restricted to controlling cell-cycle
      progression but may include the ubiquitination of yet unidentified substrates in 
      differentiated cells.
FAU - Gieffers, C
AU  - Gieffers C
AD  - Research Institute of Molecular Pathology, Dr. Bohr Gasse 7, A-1030 Vienna,
      Austria.
FAU - Peters, B H
AU  - Peters BH
FAU - Kramer, E R
AU  - Kramer ER
FAU - Dotti, C G
AU  - Dotti CG
FAU - Peters, J M
AU  - Peters JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Antigens, CD)
RN  - 0 (Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome)
RN  - 0 (CDC20 protein, S cerevisiae)
RN  - 0 (CDC27 protein, S cerevisiae)
RN  - 0 (CDC27 protein, human)
RN  - 0 (CDH1 protein, S cerevisiae)
RN  - 0 (CDH1 protein, human)
RN  - 0 (Cadherins)
RN  - 0 (Cdc20 Proteins)
RN  - 0 (Cdh1 Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Ubiquitins)
RN  - EC 2.3.2.23 (Ubiquitin-Protein Ligase Complexes)
RN  - EC 2.3.2.27 (Anaphase-Promoting Complex-Cyclosome)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 6.- (Ligases)
SB  - IM
MH  - Anaphase
MH  - Anaphase-Promoting Complex-Cyclosome
MH  - Animals
MH  - Antigens, CD
MH  - Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome
MH  - Brain Chemistry
MH  - Cadherins
MH  - Cdc20 Proteins
MH  - Cdh1 Proteins
MH  - Cell Cycle Proteins/analysis
MH  - Cell Differentiation
MH  - Fungal Proteins/*analysis
MH  - HeLa Cells
MH  - Humans
MH  - Ligases/*analysis/chemistry/physiology
MH  - Mice
MH  - Neurons/*chemistry
MH  - Rats
MH  - *Saccharomyces cerevisiae Proteins
MH  - *Ubiquitin-Protein Ligase Complexes
MH  - Ubiquitin-Protein Ligases
MH  - Ubiquitins/metabolism
PMC - PMC18031
EDAT- 1999/09/29 00:00
MHDA- 1999/09/29 00:01
CRDT- 1999/09/29 00:00
PHST- 1999/09/29 00:00 [pubmed]
PHST- 1999/09/29 00:01 [medline]
PHST- 1999/09/29 00:00 [entrez]
AID - 10.1073/pnas.96.20.11317 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11317-22. doi:
      10.1073/pnas.96.20.11317.