PMID- 10500157
OWN - NLM
STAT- MEDLINE
DCOM- 19991021
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 20
DP  - 1999 Sep 28
TI  - Estrogen induction of the cyclin D1 promoter: involvement of a cAMP response-like
      element.
PG  - 11217-22
AB  - Estrogens induce cell proliferation in target tissues by stimulating progression 
      through the G(1) phase of the cell cycle. Induction of cyclin D1 expression is a 
      critical feature of the mitogenic action of estrogen. We have determined a region
      between -96 and -29 in the cyclin D1 promoter that confers regulation by
      estrogens in the human mammary carcinoma cells MCF-7. This region encompasses a
      unique known transcription factor binding site with a sequence of a potential
      cAMP response element (CRE-D1). The induction is strictly hormone dependent and
      requires the DNA binding domain as well as both AF-1 and AF-2 domains of the
      estrogen receptor (ER) alpha. Destruction of the CRE-D1 motif caused complete
      loss of estrogen responsiveness. Both c-Jun and ATF-2 transactivated the cyclin
      D1 promoter in transient transfection experiments, and a clear additional
      increase was detected when ER was cotransfected with either c-Jun or with c-Jun
      and ATF-2 but not with ATF-2 alone. Furthermore, the expression of a dominant
      negative variant of c-Jun, TAM67, completely abolished the induction of the
      cyclin D1 promoter both in the absence and presence of ER. We show that ATF-2
      homodimers and ATF-2/c-Jun heterodimers, but not c-Jun homodimers, were able to
      bind the CRE of the cyclin D1 promoter. To interpret these results, we propose a 
      mechanism in which ATF-2/c-Jun heterodimers bind to the CRE-D1 element and
      mediate the activation of cyclin D1 promoter by the ER. This mechanism represents
      a pathway by which estrogens control the proliferation of target cells.
FAU - Sabbah, M
AU  - Sabbah M
AD  - Institut National de la Sante et de la Recherche Medicale U482, Hopital
      Saint-Antoine, 184 Rue du Faubourg Saint-Antoine, 75571 Paris Cedex 12, France.
FAU - Courilleau, D
AU  - Courilleau D
FAU - Mester, J
AU  - Mester J
FAU - Redeuilh, G
AU  - Redeuilh G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (ATF2 protein, human)
RN  - 0 (Activating Transcription Factor 2)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Estrogens)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Transcription Factors)
RN  - 136601-57-5 (Cyclin D1)
RN  - E0399OZS9N (Cyclic AMP)
SB  - IM
MH  - Activating Transcription Factor 2
MH  - Cyclic AMP/*pharmacology
MH  - Cyclic AMP Response Element-Binding Protein/metabolism
MH  - Cyclin D1/*genetics
MH  - Estrogens/*pharmacology
MH  - HeLa Cells
MH  - Humans
MH  - *Promoter Regions, Genetic
MH  - Proto-Oncogene Proteins c-jun/metabolism
MH  - Receptors, Estrogen/metabolism
MH  - *Response Elements
MH  - Transcription Factors/metabolism
PMC - PMC18014
EDAT- 1999/09/29 00:00
MHDA- 1999/09/29 00:01
CRDT- 1999/09/29 00:00
PHST- 1999/09/29 00:00 [pubmed]
PHST- 1999/09/29 00:01 [medline]
PHST- 1999/09/29 00:00 [entrez]
AID - 10.1073/pnas.96.20.11217 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11217-22. doi:
      10.1073/pnas.96.20.11217.