PMID- 10500093
OWN - NLM
STAT- MEDLINE
DCOM- 19991025
LR  - 20190516
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 13
IP  - 18
DP  - 1999 Sep 15
TI  - Ubiquitous expression and embryonic requirement for RNA polymerase II coactivator
      subunit Srb7 in mice.
PG  - 2365-8
AB  - Mammalian RNA polymerase II complexes and coactivators containing homologs of
      yeast Srb/Med proteins have been isolated recently from tissue culture cells. The
      yeast Srb/Med complex is involved in global gene expression and is essential, but
      it is not yet known if its mammalian counterparts are broadly expressed in
      tissues or if they are essential. We have isolated the murine gene encoding Srb7,
      an Srb/Med complex protein whose sequence and function is highly conserved
      between yeast and humans. The mouse Srb7 gene is single copy, and Northern
      analysis showed that it is expressed in all tissues examined. Disruption of the
      gene in embryonic stem cells revealed that it is essential for cell viability and
      murine embryonic development. These results, together with evidence that murine
      Srb7 is associated exclusively with high molecular weight forms of RNA polymerase
      II in extracts, suggest that Srb7-containing polymerase complexes occur in most
      tissues and have essential roles in expression of protein coding genes.
FAU - Tudor, M
AU  - Tudor M
AD  - Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142 USA.
FAU - Murray, P J
AU  - Murray PJ
FAU - Onufryk, C
AU  - Onufryk C
FAU - Jaenisch, R
AU  - Jaenisch R
FAU - Young, R A
AU  - Young RA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (DNA, Complementary)
RN  - 0 (Surb7 protein, mouse)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Northern
MH  - DNA, Complementary/analysis
MH  - Embryo, Mammalian/metabolism
MH  - Gene Expression Regulation, Developmental
MH  - *Gene Expression Regulation, Enzymologic
MH  - Gene Library
MH  - Humans
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - Mutagenesis, Insertional
MH  - Sequence Homology, Amino Acid
MH  - Time Factors
MH  - Tissue Distribution
MH  - Transcription Factors/*genetics/*metabolism/physiology
PMC - PMC317028
EDAT- 1999/09/29 00:00
MHDA- 1999/09/29 00:01
CRDT- 1999/09/29 00:00
PHST- 1999/09/29 00:00 [pubmed]
PHST- 1999/09/29 00:01 [medline]
PHST- 1999/09/29 00:00 [entrez]
AID - 10.1101/gad.13.18.2365 [doi]
PST - ppublish
SO  - Genes Dev. 1999 Sep 15;13(18):2365-8. doi: 10.1101/gad.13.18.2365.