PMID- 10499800
OWN - NLM
STAT- MEDLINE
DCOM- 19991015
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 98
IP  - 6
DP  - 1999 Sep 17
TI  - Recognition of polyadenylate RNA by the poly(A)-binding protein.
PG  - 835-45
AB  - The cocrystal structure of human poly(A)-binding protein (PABP) has been
      determined at 2.6 A resolution. PABP recognizes the 3' mRNA poly(A) tail and
      plays critical roles in eukaryotic translation initiation and mRNA
      stabilization/degradation. The minimal PABP used in this study consists of the
      N-terminal two RRM-type RNA-binding domains connected by a short linker (RRM1/2).
      These two RRMs form a continuous RNA-binding trough, lined by an antiparallel
      beta sheet backed by four alpha helices. The polyadenylate RNA adopts an extended
      conformation running the length of the molecular trough. Adenine recognition is
      primarily mediated by contacts with conserved residues found in the RNP motifs of
      the two RRMs. The convex dorsum of RRM1/2 displays a phylogenetically conserved
      hydrophobic/acidic portion, which may interact with translation initiation
      factors and regulatory proteins.
FAU - Deo, R C
AU  - Deo RC
AD  - Laboratories of Molecular Biophysics, The Rockefeller University, New York, New
      York 10021, USA.
FAU - Bonanno, J B
AU  - Bonanno JB
FAU - Sonenberg, N
AU  - Sonenberg N
FAU - Burley, S K
AU  - Burley SK
LA  - eng
SI  - PDB/1CVJ
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Peptide Fragments)
RN  - 0 (Poly(A)-Binding Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 24937-83-5 (Poly A)
RN  - JAC85A2161 (Adenine)
SB  - IM
MH  - Adenine/chemistry
MH  - Amino Acid Sequence
MH  - Conserved Sequence
MH  - Crystallography, X-Ray
MH  - Humans
MH  - Hydrogen Bonding
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Peptide Fragments/chemistry/genetics
MH  - Poly A/*chemistry
MH  - Poly(A)-Binding Proteins
MH  - Protein Binding
MH  - Protein Conformation
MH  - RNA, Messenger/*chemistry
MH  - RNA-Binding Proteins/*chemistry/genetics
MH  - Recombinant Proteins/chemistry
MH  - Sequence Homology, Amino Acid
EDAT- 1999/09/28 00:00
MHDA- 1999/09/28 00:01
CRDT- 1999/09/28 00:00
PHST- 1999/09/28 00:00 [pubmed]
PHST- 1999/09/28 00:01 [medline]
PHST- 1999/09/28 00:00 [entrez]
AID - S0092-8674(00)81517-2 [pii]
AID - 10.1016/s0092-8674(00)81517-2 [doi]
PST - ppublish
SO  - Cell. 1999 Sep 17;98(6):835-45. doi: 10.1016/s0092-8674(00)81517-2.