PMID- 10499587
OWN - NLM
STAT- MEDLINE
DCOM- 19991014
LR  - 20131121
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 401
IP  - 6750
DP  - 1999 Sep 16
TI  - Human urotensin-II is a potent vasoconstrictor and agonist for the orphan
      receptor GPR14.
PG  - 282-6
AB  - Urotensin-II (U-II) is a vasoactive 'somatostatin-like' cyclic peptide which was 
      originally isolated from fish spinal cords, and which has recently been cloned
      from man. Here we describe the identification of an orphan human
      G-protein-coupled receptor homologous to rat GPR14 and expressed predominantly in
      cardiovascular tissue, which functions as a U-II receptor. Goby and human U-II
      bind to recombinant human GPR14 with high affinity, and the binding is
      functionally coupled to calcium mobilization. Human U-II is found within both
      vascular and cardiac tissue (including coronary atheroma) and effectively
      constricts isolated arteries from non-human primates. The potency of
      vasoconstriction of U-II is an order of magnitude greater than that of
      endothelin-1, making human U-II the most potent mammalian vasoconstrictor
      identified so far. In vivo, human U-II markedly increases total peripheral
      resistance in anaesthetized non-human primates, a response associated with
      profound cardiac contractile dysfunction. Furthermore, as U-II immunoreactivity
      is also found within central nervous system and endocrine tissues, it may have
      additional activities.
FAU - Ames, R S
AU  - Ames RS
AD  - Department of Molecular Biology, Smith Kline Beecham Pharmaceuticals, King of
      Prussia, Pennsylvania 19406-0939, USA. bobvames1@sbphrd.com
FAU - Sarau, H M
AU  - Sarau HM
FAU - Chambers, J K
AU  - Chambers JK
FAU - Willette, R N
AU  - Willette RN
FAU - Aiyar, N V
AU  - Aiyar NV
FAU - Romanic, A M
AU  - Romanic AM
FAU - Louden, C S
AU  - Louden CS
FAU - Foley, J J
AU  - Foley JJ
FAU - Sauermelch, C F
AU  - Sauermelch CF
FAU - Coatney, R W
AU  - Coatney RW
FAU - Ao, Z
AU  - Ao Z
FAU - Disa, J
AU  - Disa J
FAU - Holmes, S D
AU  - Holmes SD
FAU - Stadel, J M
AU  - Stadel JM
FAU - Martin, J D
AU  - Martin JD
FAU - Liu, W S
AU  - Liu WS
FAU - Glover, G I
AU  - Glover GI
FAU - Wilson, S
AU  - Wilson S
FAU - McNulty, D E
AU  - McNulty DE
FAU - Ellis, C E
AU  - Ellis CE
FAU - Elshourbagy, N A
AU  - Elshourbagy NA
FAU - Shabon, U
AU  - Shabon U
FAU - Trill, J J
AU  - Trill JJ
FAU - Hay, D W
AU  - Hay DW
FAU - Ohlstein, E H
AU  - Ohlstein EH
FAU - Bergsma, D J
AU  - Bergsma DJ
FAU - Douglas, S A
AU  - Douglas SA
LA  - eng
SI  - GENBANK/AF140630
SI  - GENBANK/AF140631
PT  - Journal Article
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (DNA, Complementary)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (UTS2R protein, human)
RN  - 0 (Urotensins)
RN  - 0 (Vasoconstrictor Agents)
RN  - 9047-55-6 (urotensin II)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - SY7Q814VUP (Calcium)
SB  - IM
EIN - Nature 1999 Dec 23-30;402(6764):898
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Calcium/metabolism
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - GTP-Binding Proteins/*agonists/genetics/*metabolism
MH  - Humans
MH  - Macaca fascicularis
MH  - Male
MH  - Molecular Sequence Data
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Cell Surface/*agonists/genetics/metabolism
MH  - *Receptors, G-Protein-Coupled
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
MH  - Urotensins/metabolism/*pharmacology
MH  - Vasoconstrictor Agents/metabolism/*pharmacology
EDAT- 1999/09/28 09:00
MHDA- 2000/03/04 09:00
CRDT- 1999/09/28 09:00
PHST- 1999/09/28 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 1999/09/28 09:00 [entrez]
AID - 10.1038/45809 [doi]
PST - ppublish
SO  - Nature. 1999 Sep 16;401(6750):282-6. doi: 10.1038/45809.