PMID- 10499420
OWN - NLM
STAT- MEDLINE
DCOM- 19991122
LR  - 20190818
IS  - 0196-9781 (Print)
IS  - 0196-9781 (Linking)
VI  - 20
IP  - 9
DP  - 1999
TI  - Identification of a Drosophila brain-gut peptide related to the neuropeptide Y 
      family.
PG  - 1035-42
AB  - A neuropeptide F (NPF) was isolated from the fruit fly, Drosophila mellanogaster, 
      based on a radioimmunoassay for a gut peptide from the corn earworm, Helicoverpa 
      zea. A partial sequence was obtained from the fly peptide, and a genomic sequence 
      coding for NPF was cloned after inverse polymerase chain reaction and shown to 
      exist as a single genomic copy. The encoded, putative prepropeptide can be 
      processed into an amidated NPF with 36 residues that is related to invertebrate 
      NPF's and the neuropeptide Y family of vertebrates. In situ hybridization and 
      immunocytochemistry showed that Drosophila NPF was expressed in the brain and 
      midgut of fly larvae and adults.
FAU - Brown, M R
AU  - Brown MR
AD  - Department of Entomology, University of Georgia, Athens 30602, USA. 
      mbrown@bugs.ent.uga.edu
FAU - Crim, J W
AU  - Crim JW
FAU - Arata, R C
AU  - Arata RC
FAU - Cai, H N
AU  - Cai HN
FAU - Chun, C
AU  - Chun C
FAU - Shen, P
AU  - Shen P
LA  - eng
GR  - AI33108/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Peptides
JT  - Peptides
JID - 8008690
RN  - 0 (Drosophila Proteins)
RN  - 0 (Neuropeptide Y)
RN  - 0 (Neuropeptides)
RN  - 0 (neuropeptide F, Drosophila)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain Chemistry
MH  - Cloning, Molecular
MH  - DNA
MH  - Drosophila Proteins
MH  - Drosophila melanogaster/*chemistry
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Intestines/chemistry
MH  - Mass Spectrometry
MH  - Molecular Sequence Data
MH  - Neuropeptide Y/*chemistry
MH  - Neuropeptides/*chemistry/genetics
MH  - Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
EDAT- 1999/09/28 00:00
MHDA- 1999/09/28 00:01
CRDT- 1999/09/28 00:00
PHST- 1999/09/28 00:00 [pubmed]
PHST- 1999/09/28 00:01 [medline]
PHST- 1999/09/28 00:00 [entrez]
AID - S0196-9781(99)00097-2 [pii]
AID - 10.1016/s0196-9781(99)00097-2 [doi]
PST - ppublish
SO  - Peptides. 1999;20(9):1035-42. doi: 10.1016/s0196-9781(99)00097-2.