PMID- 10498869
OWN - NLM
STAT- MEDLINE
DCOM- 19991021
LR  - 20071115
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 37
DP  - 1999 Sep 16
TI  - Conserved BRCT regions of TopBP1 and of the tumor suppressor BRCA1 bind strand
      breaks and termini of DNA.
PG  - 5194-203
AB  - The BRCT region, the carboxyl-terminus of BRCA1 (the breast cancer susceptibility
      gene 1 product), is a ubiquitous region homologous to regions in DNA repair
      enzymes and cell cycle regulators. We showed that the BRCT regions bound DNA
      fragments, using the TopBP1 protein (topoisomerase II binding protein 1), with
      eight BRCTs as a model protein. The bindings were independent of DNA sequences,
      forms of DNA termini and energy. The BRCT-DNA complex showed resistance to an
      exonuclease, indicating that BRCT bound DNA breaks. The BRCTs also bound DNA
      nicks, suggesting that BRCTs play an important role in detection of both single- 
      and double-strand DNA breaks or ends. On the other hand, BRCTs did not bind
      circular intact DNA. BRCTs of BRCA1 also bound DNA termini. Since some BRCTs are 
      unique general elements in some tumor suppressions, these findings will reveal
      novel aspects of the tumor suppression mechanism.
FAU - Yamane, K
AU  - Yamane K
AD  - Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1,
      Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
FAU - Tsuruo, T
AU  - Tsuruo T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (BRCA1 Protein)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Circular)
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (TOPBP1 protein, human)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - BRCA1 Protein/chemistry/*metabolism
MH  - Carrier Proteins/chemistry/*metabolism
MH  - Cell Cycle
MH  - Consensus Sequence
MH  - DNA/metabolism/radiation effects
MH  - DNA Damage
MH  - DNA Repair
MH  - DNA, Circular/metabolism
MH  - DNA, Single-Stranded/metabolism
MH  - DNA-Binding Proteins
MH  - Evolution, Molecular
MH  - Humans
MH  - Nuclear Proteins
MH  - Oligonucleotides, Antisense/pharmacology
MH  - Protein Binding
MH  - Recombinant Fusion Proteins/metabolism
MH  - Structure-Activity Relationship
MH  - Substrate Specificity
MH  - Ultraviolet Rays
EDAT- 1999/09/28 00:00
MHDA- 1999/09/28 00:01
CRDT- 1999/09/28 00:00
PHST- 1999/09/28 00:00 [pubmed]
PHST- 1999/09/28 00:01 [medline]
PHST- 1999/09/28 00:00 [entrez]
AID - 10.1038/sj.onc.1202922 [doi]
PST - ppublish
SO  - Oncogene. 1999 Sep 16;18(37):5194-203. doi: 10.1038/sj.onc.1202922.