PMID- 10498862
OWN - NLM
STAT- MEDLINE
DCOM- 19991021
LR  - 20161124
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 37
DP  - 1999 Sep 16
TI  - Identification of human APC10/Doc1 as a subunit of anaphase promoting complex.
PG  - 5131-7
AB  - Anaphase-promoting complex or cyclosome (APC) is a ubiquitin ligase which
      specifically targets mitotic regulatory factors such as Pds1/Cut2 and cyclin B.
      Identification of the subunits of multiprotein complex APC in several species
      revealed the highly conserved composition of APC from yeast to human. It has been
      reported, however, that vertebrate APC is composed of at least eight subunits,
      APC1 to APC8, while budding yeast APC is constituted of at least 12 components,
      Apc1 to Apc13. It has not yet been clearly understood whether additional
      components found in budding yeast, Apc9 to Apc13, are actually composed of
      mammalian APC. Here we isolated and characterized human APC10/Doc1, and found
      that APC10/Doc1 binds to APC core subunits throughout the cell cycle. Further, it
      was found that APC10/Doc1 is localized in centrosomes and mitotic spindles
      throughout mitosis, while it is also localized in kinetochores from prophase to
      anaphase and in midbody in telophase and cytokinesis. These results strongly
      support the notion that human APC10/Doc1 may be one of the APC core subunits
      rather than the transiently associated regulatory factor.
FAU - Kurasawa, Y
AU  - Kurasawa Y
AD  - Tsukuba Life Science Center, The Institute of Physical and Chemical Research
      (RIKEN), 3-1, Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
FAU - Todokoro, K
AU  - Todokoro K
LA  - eng
SI  - GENBANK/AB012109
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (ANAPC1 protein, human)
RN  - 0 (Apc1 Subunit, Anaphase-Promoting Complex-Cyclosome)
RN  - 0 (Apc10 Subunit, Anaphase-Promoting Complex-Cyclosome)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (DOC1 protein, S cerevisiae)
RN  - 0 (Fungal Proteins)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 2.3.2.23 (Ubiquitin-Protein Ligase Complexes)
RN  - EC 2.3.2.27 (Anaphase-Promoting Complex-Cyclosome)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 6.- (Ligases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Anaphase/*physiology
MH  - Anaphase-Promoting Complex-Cyclosome
MH  - Apc1 Subunit, Anaphase-Promoting Complex-Cyclosome
MH  - Apc10 Subunit, Anaphase-Promoting Complex-Cyclosome
MH  - Cell Cycle
MH  - Cell Cycle Proteins/isolation & purification/*physiology
MH  - Centromere/chemistry
MH  - Centrosome/chemistry
MH  - DNA, Complementary/genetics
MH  - Fungal Proteins/physiology
MH  - HeLa Cells
MH  - Humans
MH  - Ligases/*chemistry
MH  - Molecular Sequence Data
MH  - Multienzyme Complexes/*chemistry
MH  - Saccharomyces cerevisiae/chemistry/cytology
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
MH  - Spindle Apparatus/chemistry
MH  - *Ubiquitin-Protein Ligase Complexes
MH  - Ubiquitin-Protein Ligases
EDAT- 1999/09/28 00:00
MHDA- 1999/09/28 00:01
CRDT- 1999/09/28 00:00
PHST- 1999/09/28 00:00 [pubmed]
PHST- 1999/09/28 00:01 [medline]
PHST- 1999/09/28 00:00 [entrez]
AID - 10.1038/sj.onc.1203133 [doi]
PST - ppublish
SO  - Oncogene. 1999 Sep 16;18(37):5131-7. doi: 10.1038/sj.onc.1203133.