PMID- 10498613
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20161124
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 94
IP  - 7
DP  - 1999 Oct 1
TI  - PTPROt: an alternatively spliced and developmentally regulated B-lymphoid
      phosphatase that promotes G0/G1 arrest.
PG  - 2403-13
AB  - Protein tyrosine phosphatases (PTP) regulate the proliferation, differentiation, 
      and viability of lymphocytes by modulating their signaling pathways. By using the
      differential display assay, we have cloned a putative receptor-type PTP, which is
      predominantly expressed in B-lymphoid tissues (lymph nodes and spleen). This PTP,
      termed PTPROt (truncated), is a tissue-specific alternatively-spliced form of a
      human epithelial PTP, PTPRO (PTPU2/GLEPP1). Whereas the epithelial PTPRO includes
      an approximately 800-amino acid extracellular domain, the major (3 kb) PTPROt
      cDNA predicts a unique 5' untranslated region and truncated (8 amino acids)
      extracellular domain with a conserved transmembrane region and single catalytic
      domain. PTPROt cDNAs encode functional approximately 47-kD and approximately
      43-kD PTPs, which are most abundant in normal naive quiescent B cells and
      decreased or absent in germinal center B cells and germinal center-derived
      diffuse large B-cell lymphomas. Because PTPROt was predominantly expressed in
      naive quiescent B cells, the enzyme's effects on cell-cycle progression were
      examined. When multiple stable PTPROt sense, antisense, and vector only B-cell
      transfectants were grown in reduced serum and synchronized with nocodazole,
      PTPROt sense clones exhibited markedly increased G0/G1 arrest. Taken together,
      these data implicate PTPROt in the growth control of specific B-cell
      subpopulations.
FAU - Aguiar, R C
AU  - Aguiar RC
AD  - Department of Adult Oncology, Dana-Farber Cancer Institute, Boston, MA, 02115,
      USA.
FAU - Yakushijin, Y
AU  - Yakushijin Y
FAU - Kharbanda, S
AU  - Kharbanda S
FAU - Tiwari, S
AU  - Tiwari S
FAU - Freeman, G J
AU  - Freeman GJ
FAU - Shipp, M A
AU  - Shipp MA
LA  - eng
SI  - GENBANK/AF152378
SI  - GENBANK/AF187042
SI  - GENBANK/AF187043
SI  - GENBANK/AF187044
GR  - CA66996/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (DNA, Complementary)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
RN  - SH1WY3R615 (Nocodazole)
SB  - AIM
SB  - IM
MH  - *Alternative Splicing
MH  - Amino Acid Sequence
MH  - B-Lymphocytes/*cytology/*enzymology
MH  - Base Sequence
MH  - Cell Cycle/drug effects/*physiology
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - DNA, Complementary
MH  - G1 Phase
MH  - Gene Expression Regulation, Developmental
MH  - Gene Expression Regulation, Enzymologic
MH  - Humans
MH  - Introns
MH  - Lymphoma, B-Cell/enzymology/genetics
MH  - Molecular Sequence Data
MH  - Nocodazole/pharmacology
MH  - Palatine Tonsil/immunology
MH  - Protein Tyrosine Phosphatases/chemistry/*genetics/metabolism
MH  - Recombinant Fusion Proteins/chemistry/metabolism
MH  - Resting Phase, Cell Cycle
MH  - Sequence Deletion
MH  - Spleen/immunology
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1999/09/25 00:00
MHDA- 1999/09/25 00:01
CRDT- 1999/09/25 00:00
PHST- 1999/09/25 00:00 [pubmed]
PHST- 1999/09/25 00:01 [medline]
PHST- 1999/09/25 00:00 [entrez]
PST - ppublish
SO  - Blood. 1999 Oct 1;94(7):2403-13.