PMID- 10498338
OWN - NLM
STAT- MEDLINE
DCOM- 19991214
LR  - 20091119
IS  - 0167-0115 (Print)
IS  - 0167-0115 (Linking)
VI  - 83
IP  - 1
DP  - 1999 Aug 31
TI  - Tissue distribution of prolactin-releasing peptide (PrRP) and its receptor.
PG  - 1-10
AB  - Prolactin-releasing peptide (PrRP) is a novel bioactive peptide, originally
      isolated from bovine hypothalamus by utilizing an orphan
      seven-transmembrane-domain receptor expressed in the human pituitary gland. In
      this paper, we analyzed the tissue distribution of rat and human PrRP and their
      receptor mRNAs by quantitative reverse transcription-polymerase chain reaction
      (RT-PCR) and Northern blotting. In RT-PCR analysis, rat PrRP receptor mRNA was
      detected in the central nervous system, and the highest expression was detected
      in the pituitary gland. In addition, in situ hybridization revealed that rat PrRP
      receptor mRNA was highly expressed in the anterior lobe of the pituitary. On the 
      other hand, rat PrRP mRNA was most abundantly expressed in the medulla oblongata,
      while significant levels of expression were widely detected in other tissues. In 
      Northern blot analyses, human PrRP receptor mRNA was detected only in the
      pituitary gland among tissues examined. Human PrRP mRNA was detected in the
      medulla oblongata and in the pancreas. In contrast to the pattern of mRNA
      expression, the highest content of bioactive PrRP was found in the hypothalamus
      rather than the medulla oblongata in the rat brain, indicating that PrRP mRNA
      does not always parallel with mature PrRP in tissue distribution. The wide
      distribution of PrRP and its receptor suggests that they have various functions
      not only in the pituitary gland but also in the other tissues.
FAU - Fujii, R
AU  - Fujii R
AD  - Discovery Research Laboratories 1, Pharmaceutical Discovery Research Division,
      Takeda Chemical Industries Ltd., Tsukuba, Ibaraki, Japan.
FAU - Fukusumi, S
AU  - Fukusumi S
FAU - Hosoya, M
AU  - Hosoya M
FAU - Kawamata, Y
AU  - Kawamata Y
FAU - Habata, Y
AU  - Habata Y
FAU - Hinuma, S
AU  - Hinuma S
FAU - Sekiguchi, M
AU  - Sekiguchi M
FAU - Kitada, C
AU  - Kitada C
FAU - Kurokawa, T
AU  - Kurokawa T
FAU - Nishimura, O
AU  - Nishimura O
FAU - Onda, H
AU  - Onda H
FAU - Sumino, Y
AU  - Sumino Y
FAU - Fujino, M
AU  - Fujino M
LA  - eng
SI  - GENBANK/AB015418
SI  - GENBANK/AB015419
SI  - GENBANK/AB015745
PT  - Journal Article
PL  - Netherlands
TA  - Regul Pept
JT  - Regulatory peptides
JID - 8100479
RN  - 0 (DNA, Complementary)
RN  - 0 (Hypothalamic Hormones)
RN  - 0 (Neuropeptides)
RN  - 0 (PRLH protein, human)
RN  - 0 (Prlh protein, rat)
RN  - 0 (Prolactin-Releasing Hormone)
RN  - 0 (Receptors, Neuropeptide)
RN  - 0 (receptor, prolactin-releasing peptide)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - DNA, Complementary
MH  - Humans
MH  - Hypothalamic Hormones/*biosynthesis/genetics
MH  - Molecular Sequence Data
MH  - Neuropeptides/*biosynthesis/genetics
MH  - Prolactin-Releasing Hormone
MH  - Rats
MH  - Receptors, Neuropeptide/*biosynthesis/genetics
MH  - Tissue Distribution
EDAT- 1999/09/25 00:00
MHDA- 1999/09/25 00:01
CRDT- 1999/09/25 00:00
PHST- 1999/09/25 00:00 [pubmed]
PHST- 1999/09/25 00:01 [medline]
PHST- 1999/09/25 00:00 [entrez]
AID - S0167-0115(99)00028-2 [pii]
PST - ppublish
SO  - Regul Pept. 1999 Aug 31;83(1):1-10.