PMID- 10498179
OWN - NLM
STAT- MEDLINE
DCOM- 19991209
LR  - 20031114
IS  - 0166-6851 (Print)
IS  - 0166-6851 (Linking)
VI  - 102
IP  - 2
DP  - 1999 Aug 20
TI  - A monoclonal antibody against a glycolipid SEGLx from Spirometra erinaceieuropaei
      plerocercoid.
PG  - 225-35
AB  - A mouse monoclonal antibody AK97 (IgM) was established against a new type of
      glycosphingolipid, SEGLx, isolated from plerocercoids of tapeworm, Spirometra
      erinaceieuropaei. The chemical structure of SEGLx (Gal beta1-4(Fuc alpha1-3)(Glc 
      beta1-3Gal beta1-ceramide) had been previously characterized. The specificity of 
      AK97 was determined by thin-layer chromatography-immunostaining and enzyme-linked
      immunosorbent assay. AK97 was found to be directed to SEGLx and GalSEGLx (Gal
      beta1-4(Fuc alpha1-3)Glc beta1-3(Gal beta1-6)Gal beta1-ceramide) and also showed 
      cross-reactivity with the stage specific embryonic antigen-1 (SSEA-1), the
      epitope being defined to be the non-reducing terminal trisaccharide sequence. On 
      immunohistochemical examination, AK97 predominantly stained the tegument, the
      external surfaces of worms which have a brush border-like organization. Based on 
      the immunohistochemical findings for the staining liability as to organic
      solvents and the results of Western blot analysis of the plerocercoid
      glycoproteins, it was proved that the antigens in the tapeworm were glycolipids. 
      Considering that the tapeworm is in direct contact with its host's tissue through
      the tegument, the membrane surface of which is exposed to the external
      environment, it is suspected that SEGLx and GalSEGLx on the tegument play
      functionally important roles in the host parasite interaction.
FAU - Yanagisawa, M
AU  - Yanagisawa M
AD  - Department of Medical Zoology, Azabu University, Sagamihara, Kanagawa, Japan.
FAU - Kojima, H
AU  - Kojima H
FAU - Kawakami, Y
AU  - Kawakami Y
FAU - Iriko, H
AU  - Iriko H
FAU - Nakamura, T
AU  - Nakamura T
FAU - Nakamura, K
AU  - Nakamura K
FAU - Uchida, A
AU  - Uchida A
FAU - Murata, Y
AU  - Murata Y
FAU - Tamai, Y
AU  - Tamai Y
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Mol Biochem Parasitol
JT  - Molecular and biochemical parasitology
JID - 8006324
RN  - 0 (Antibodies, Helminth)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes)
RN  - 0 (Glycosphingolipids)
SB  - IM
MH  - Animals
MH  - Antibodies, Helminth/immunology
MH  - Antibodies, Monoclonal/*immunology
MH  - Antibody Specificity
MH  - Blotting, Western
MH  - Carbohydrate Sequence
MH  - Chromatography, Thin Layer
MH  - Electrophoresis, Agar Gel
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epitopes
MH  - Female
MH  - Glycosphingolipids/*analysis/chemistry/*immunology
MH  - Hybridomas
MH  - Immunohistochemistry
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - Spirometra/*chemistry/growth & development
EDAT- 1999/09/25 00:00
MHDA- 1999/09/25 00:01
CRDT- 1999/09/25 00:00
PHST- 1999/09/25 00:00 [pubmed]
PHST- 1999/09/25 00:01 [medline]
PHST- 1999/09/25 00:00 [entrez]
AID - S0166-6851(99)00102-4 [pii]
PST - ppublish
SO  - Mol Biochem Parasitol. 1999 Aug 20;102(2):225-35.