PMID- 10497345
OWN - NLM
STAT- MEDLINE
DCOM- 19991021
LR  - 20061115
IS  - 1040-452X (Print)
IS  - 1040-452X (Linking)
VI  - 54
IP  - 3
DP  - 1999 Nov
TI  - Developmental stage- and germ cell-regulated expression of a calcium-binding
      protein mRNA in mouse Sertoli cells.
PG  - 232-43
AB  - There is considerable evidence that germ cells, mainly spermatocytes and
      spermatids, contribute to the regulation of Sertoli cell activity. We developed
      an in vitro system to investigate the genes involved in Sertoli cell-germ cell
      interaction in the mouse by using the differential mRNA display technique. One of
      the isolated differentially expressed genes, named calgizzarin, belongs to the
      family of S100 calcium-binding proteins and shows a decreased expression in
      Sertoli cell-germ cell cocultures compared to cultured Sertoli cells alone.
      Calgizzarin is expressed in all adult tissues examined, including testis and
      ovary; however, a high mRNA level for calgizzarin in mouse testis is maintained
      until day 15 of postnatal development and then declines dramatically, whereas the
      expression pattern in the ovary remains constantly high. Furthermore, Northern
      blot studies on testicular RNA from different mouse mutants with defects in
      spermatogenesis revealed that high levels of calgizzarin transcripts can only be 
      detected in testes of mouse mutants with either no germ cells or primary
      spermatocytes, but only weak signals for calgizzarin are observed in testes of
      mutants containing spermatids. In addition, using both RT-PCR analysis and
      whole-mount in situ hybridization on dissected gonads it was demonstrated that
      mouse calgizzarin expression starts at 13.5 dpc in the prenatal male gonad and at
      16.5 dpc in the embryonic ovary, respectively. The mouse calgizzarin gene was
      localized on mouse chromosome 5, region E-F. Taken together, our results indicate
      that calgizzarin expression could be repressed by factors originated from
      pachytene spermatocytes and/or spermatids.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - Kraszucka, K
AU  - Kraszucka K
AD  - Institute of Human Genetics, University of Gottingen, Gottingen, Germany.
FAU - Burfeind, P
AU  - Burfeind P
FAU - Nayernia, K
AU  - Nayernia K
FAU - Kohler, M
AU  - Kohler M
FAU - Schmid, M
AU  - Schmid M
FAU - Yaylaoglu, M
AU  - Yaylaoglu M
FAU - Engel, W
AU  - Engel W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (CAL protein, Arabidopsis)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (MADS Domain Proteins)
RN  - 0 (Plant Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Arabidopsis Proteins
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cell Line
MH  - Chromosome Mapping
MH  - DNA, Complementary/metabolism
MH  - DNA-Binding Proteins/genetics
MH  - Down-Regulation
MH  - Exons
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - Germ Cells/*metabolism
MH  - In Situ Hybridization
MH  - Introns
MH  - *MADS Domain Proteins
MH  - Male
MH  - Meiosis
MH  - Mice
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - Ovary/embryology
MH  - Plant Proteins/genetics
MH  - RNA, Messenger/*analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
MH  - Sertoli Cells/*metabolism
MH  - Spermatocytes/metabolism
MH  - Testis/embryology
EDAT- 1999/09/25 00:00
MHDA- 1999/09/25 00:01
CRDT- 1999/09/25 00:00
PHST- 1999/09/25 00:00 [pubmed]
PHST- 1999/09/25 00:01 [medline]
PHST- 1999/09/25 00:00 [entrez]
AID - 10.1002/(SICI)1098-2795(199911)54:3<232::AID-MRD4>3.0.CO;2-F [pii]
AID - 10.1002/(SICI)1098-2795(199911)54:3<232::AID-MRD4>3.0.CO;2-F [doi]
PST - ppublish
SO  - Mol Reprod Dev. 1999 Nov;54(3):232-43. doi:
      10.1002/(SICI)1098-2795(199911)54:3<232::AID-MRD4>3.0.CO;2-F.