PMID- 10497269
OWN - NLM
STAT- MEDLINE
DCOM- 19991217
LR  - 20190501
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 27
IP  - 20
DP  - 1999 Oct 15
TI  - AP2alpha and AP2gamma: a comparison of binding site specificity and
      trans-activation of the estrogen receptor promoter and single site promoter
      constructs.
PG  - 4040-9
AB  - The AP2 transcription factors exhibit a high degree of homology in the DNA
      binding and dimerization domains. In this study, we methodically compared the
      binding specificity of AP2alpha and AP2gamma using PCR-assisted binding site
      selection and competitive gel shift assay and determined that the consensus
      binding site for both factors is(G)/(C)CCNN(A/)C(/G)(G)/(A)G(G/)C(/T.)The use of 
      single site promoter constructs with either a high or low affinity site
      demonstrated a direct relationship between site affinity and transcriptional
      activation. Overexpression of AP2alpha and AP2gamma resulted in the activation of
      a low affinity binding site construct to levels comparable to those seen with a
      high affinity site construct at lower amounts of protein expression. Both
      AP2alpha and AP2gamma were able to trans-activate the cloned human estrogen
      receptor alpha promoter in ER-negative MDA-MB-231 cells through high affinity AP2
      sites in the untranslated leader sequence. This provides a functional mechanism
      to explain the correlation between AP2 activity and estrogen receptor expression 
      in breast cancer. Since there is overexpression of AP2 factors in breast cancer
      compared to normal breast epithelium, our results suggest that increased factor
      expression may activate a set of target genes containing lower affinity binding
      sites that would normally not be expressed in normal breast epithelium.
FAU - McPherson, L A
AU  - McPherson LA
AD  - Department of Surgery, Stanford University, MSLS, Room P214, Stanford, CA
      94305-5408, USA.
FAU - Weigel, R J
AU  - Weigel RJ
LA  - eng
GR  - 1R29CA63251/CA/NCI NIH HHS/United States
GR  - 1RO1CA77350/CA/NCI NIH HHS/United States
GR  - 5-T32-DK07217-21/DK/NIDDK NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Transcription Factor AP-2)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Binding Sites
MH  - Cell Line
MH  - DNA-Binding Proteins/*metabolism
MH  - Humans
MH  - Polymerase Chain Reaction
MH  - *Promoter Regions, Genetic
MH  - Receptors, Estrogen/*genetics
MH  - Transcription Factor AP-2
MH  - Transcription Factors/*metabolism
MH  - *Transcriptional Activation
PMC - PMC148672
EDAT- 1999/09/25 00:00
MHDA- 1999/09/25 00:01
CRDT- 1999/09/25 00:00
PHST- 1999/09/25 00:00 [pubmed]
PHST- 1999/09/25 00:01 [medline]
PHST- 1999/09/25 00:00 [entrez]
AID - gkc602 [pii]
AID - 10.1093/nar/27.20.4040 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1999 Oct 15;27(20):4040-9. doi: 10.1093/nar/27.20.4040.