PMID- 10497265
OWN - NLM
STAT- MEDLINE
DCOM- 19991217
LR  - 20190501
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 27
IP  - 20
DP  - 1999 Oct 15
TI  - Identification of differentially expressed genes associated with HER-2/neu
      overexpression in human breast cancer cells.
PG  - 4008-17
AB  - Amplification and resulting overexpression of the HER-2/ neu proto-oncogene is
      found in approximately 30% of human breast and 20% of human ovarian cancers. To
      better understand the molecular events associated with overexpression of this
      gene in human breast cancer cells, differential hybridization was used to
      identify genes whose expression levels are altered in cells overexpressing this
      receptor. Of 16 000 clones screened from an overexpression cell cDNA library, a
      total of 19 non-redundant clones were isolated including seven whose expression
      decreases (C clones) and 12 which increase (H clones) in association with HER-2/ 
      neu overexpression. Of these, five C clones and 11 H clones have been confirmed
      to be differentially expressed by northern blot analysis. This group includes
      nine genes of known function, three previously sequenced genes of relatively
      uncharacterized function and four novel genes without a match in GenBank.
      Examination of the previously characterized genes indicates that they represent
      sequences known to be frequently associated with the malignant phenotype,
      suggesting that the subtraction cloning strategy used identified appropriate
      target genes. In addition, differential expression of 12 of 16 (75%) cDNAs
      identified in the breast cancer cell lines are also seen in HER-2/ neu
      -overexpressing ovarian cancer cells, indicating that they represent generic
      associations with HER-2/ neu overexpression. Finally, up-regulation of two of the
      identified cDNAs, one novel and one identified but as yet uncharacterized gene,
      was confirmed in human breast cancer specimens in association with HER-2/ neu
      overexpression. Further characterization of these genes may yield insight into
      the fundamental biology and pathogenetic effects of HER-2/ neu overexpression in 
      human breast and ovarian cancer cells.
FAU - Oh, J J
AU  - Oh JJ
AD  - Department of Medicine, Division of Hematology and Oncology, UCLA School of
      Medicine, Los Angeles, CA 90095-1678, USA.
FAU - Grosshans, D R
AU  - Grosshans DR
FAU - Wong, S G
AU  - Wong SG
FAU - Slamon, D J
AU  - Slamon DJ
LA  - eng
SI  - GENBANK/AF103798
SI  - GENBANK/AF103799
SI  - GENBANK/AF103800
SI  - GENBANK/AF103801
SI  - GENBANK/AF103802
SI  - GENBANK/AF103803
SI  - GENBANK/AF103804
GR  - P01 CA32737/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Amino Acid Sequence
MH  - Blotting, Northern
MH  - Breast Neoplasms/genetics/*metabolism
MH  - Female
MH  - Gene Amplification
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Molecular Sequence Data
MH  - Ovarian Neoplasms/genetics/metabolism
MH  - Phenotype
MH  - Receptor, ErbB-2/*biosynthesis/genetics
MH  - Tumor Cells, Cultured
PMC - PMC148668
EDAT- 1999/09/25 00:00
MHDA- 1999/09/25 00:01
CRDT- 1999/09/25 00:00
PHST- 1999/09/25 00:00 [pubmed]
PHST- 1999/09/25 00:01 [medline]
PHST- 1999/09/25 00:00 [entrez]
AID - gkc578 [pii]
AID - 10.1093/nar/27.20.4008 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1999 Oct 15;27(20):4008-17. doi: 10.1093/nar/27.20.4008.