PMID- 10497219
OWN - NLM
STAT- MEDLINE
DCOM- 19991102
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 40
DP  - 1999 Oct 1
TI  - Novel rabphilin-3-like protein associates with insulin-containing granules in
      pancreatic beta cells.
PG  - 28542-8
AB  - A novel rabphilin-3-like gene, granuphilin, has been identified in pancreatic
      beta cells by comparing genes expressed in pancreatic alpha and beta cell lines
      using mRNA differential display. The domain structure of the protein products of 
      the granuphilin gene contains an amino-terminal zinc-finger motif and
      carboxyl-terminal C(2)-domains, similar to that of the rabphilin-3 gene. There
      are two isoforms: the larger isoform, granuphilin-a, has two C(2)-domains,
      whereas the smaller one, granuphilin-b, contains only the first C(2)-domain.
      Granuphilin is specifically expressed in pancreatic beta cells and the pituitary 
      gland, but not in pancreatic alpha cells, the adrenal gland, or other major
      organs such as the brain. A portion of granuphilin associates with
      insulin-containing dense-core granules, but not with synaptic-like microvesicles 
      in beta cells. Thus, its distribution pattern presents a striking contrast with
      that of rabphilin-3, which associates with small synaptic vesicles in neurons.
      The first C(2)-domain of granuphilin binds phospholipids in a Ca(2+)-independent 
      manner, whereas the second one does not. These distinctive characteristics of
      granuphilin suggest that it is not a simple counterpart of rabphilin-3 in
      endocrine cells and that it has a unique role in the regulated exocytosis of
      dense-core granules in endocrine tissues.
FAU - Wang, J
AU  - Wang J
AD  - Department of Molecular Medicine, Institute for Molecular and Cellular
      Regulation, Gunma University, Maebashi 371-8512, Japan.
FAU - Takeuchi, T
AU  - Takeuchi T
FAU - Yokota, H
AU  - Yokota H
FAU - Izumi, T
AU  - Izumi T
LA  - eng
SI  - GENBANK/AB025258
SI  - GENBANK/AB025259
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Insulin)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Phospholipids)
RN  - 0 (Sytl4 protein, mouse)
RN  - 0 (Vesicular Transport Proteins)
RN  - 0 (rabphilin-3A)
RN  - EC 3.6.5.2 (rab GTP-Binding Proteins)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Calcium/metabolism
MH  - Carrier Proteins/chemistry/genetics/*metabolism
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Cytoplasmic Granules/*metabolism
MH  - DNA, Complementary
MH  - Insulin/*metabolism
MH  - Islets of Langerhans/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/chemistry/*metabolism
MH  - Phospholipids/metabolism
MH  - Protein Binding
MH  - Sequence Homology, Amino Acid
MH  - Vesicular Transport Proteins
MH  - rab GTP-Binding Proteins/chemistry/*metabolism
EDAT- 1999/09/25 00:00
MHDA- 1999/09/25 00:01
CRDT- 1999/09/25 00:00
PHST- 1999/09/25 00:00 [pubmed]
PHST- 1999/09/25 00:01 [medline]
PHST- 1999/09/25 00:00 [entrez]
AID - 10.1074/jbc.274.40.28542 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Oct 1;274(40):28542-8. doi: 10.1074/jbc.274.40.28542.