PMID- 10496535
OWN - NLM
STAT- MEDLINE
DCOM- 19991007
LR  - 20171213
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 79
IP  - 9
DP  - 1999 Sep
TI  - PDZK1, a novel PDZ domain-containing protein up-regulated in carcinomas and
      mapped to chromosome 1q21, interacts with cMOAT (MRP2), the multidrug
      resistance-associated protein.
PG  - 1161-70
AB  - We recently reported the isolation and partial characterization of two novel
      proteins, MAP17 and PDZK1. Using in situ hybridization, we demonstrated that
      MAP17 and PDZK1 mRNAs are markedly up-regulated in human carcinomas. PDZK1,
      originally isolated as a protein interacting with MAP17, contains four PDZ
      protein-interaction domains and could potentially interact with as many as four
      target proteins. In this paper, we confirm the overexpression of PDZK1 in human
      carcinomas using a specific antibody and demonstrate the localization of the
      PDZK1 gene to human chromosome 1q21, a region frequently altered in neoplastic
      conditions. Using the yeast two-hybrid system, we have also determined that PDZK1
      interacts with the carboxy-terminal portion of cMOAT (MRP2), the canalicular
      multispecific organic anion transporter associated with multidrug resistance.
      This is of particular interest because proteins containing PDZ domains are
      involved in the clustering and signaling pathways of membrane-associated
      proteins, including ion channels. Therefore, the protein cluster formed by the
      association of cMOAT, PDZK1, and MAP17 could play an important role in the
      cellular mechanisms associated with multidrug resistance, and PDZK1 may represent
      a new target in cancer cells resistant to chemotherapeutic agents.
FAU - Kocher, O
AU  - Kocher O
AD  - Department of Pathology, Beth Israel-Deaconess Medical Center and Harvard Medical
      School, Boston, Massachusetts 02215, USA. okocher@caregroup.harvard.edu
FAU - Comella, N
AU  - Comella N
FAU - Gilchrist, A
AU  - Gilchrist A
FAU - Pal, R
AU  - Pal R
FAU - Tognazzi, K
AU  - Tognazzi K
FAU - Brown, L F
AU  - Brown LF
FAU - Knoll, J H
AU  - Knoll JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Anion Transport Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Multidrug Resistance-Associated Proteins)
SB  - IM
MH  - ATP-Binding Cassette Transporters/*genetics/metabolism
MH  - Anion Transport Proteins
MH  - Blotting, Northern
MH  - Carcinoma/*genetics/metabolism
MH  - Carrier Proteins/*genetics/metabolism
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 1
MH  - *Drug Resistance, Multiple
MH  - Humans
MH  - In Situ Hybridization
MH  - Membrane Proteins/*genetics/metabolism
MH  - Multidrug Resistance-Associated Proteins
MH  - *Protein Structure, Tertiary
MH  - Up-Regulation
EDAT- 1999/09/25 00:00
MHDA- 1999/09/25 00:01
CRDT- 1999/09/25 00:00
PHST- 1999/09/25 00:00 [pubmed]
PHST- 1999/09/25 00:01 [medline]
PHST- 1999/09/25 00:00 [entrez]
PST - ppublish
SO  - Lab Invest. 1999 Sep;79(9):1161-70.