PMID- 10496312
OWN - NLM
STAT- MEDLINE
DCOM- 19991004
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 66
IP  - 3
DP  - 1999 Sep
TI  - Ubiquicidin, a novel murine microbicidal protein present in the cytosolic
      fraction of macrophages.
PG  - 423-8
AB  - Previously we have identified and characterized three murine microbicidal
      proteins purified from the granule fraction of cells from the murine macrophage
      cell line RAW264.7. During these studies evidence was obtained for the presence
      of an additional antimicrobial protein in the cytosolic fraction of RAW264.7
      cells that had been activated with interferon-gamma (IFN-gamma). In this study we
      have purified this protein, designated ubiquicidin, to apparent homogeneity and
      demonstrated that it is a cationic, small (Mr 6654) protein. Ubiquicidin
      displayed marked antimicrobial activity against Listeria monocytogenes and
      Salmonella typhimurium. Using a gel overlay procedure evidence was obtained that 
      the protein also displays activity against Escherichia coli, Staphylococcus
      aureus, and an avirulent strain of Yersinia enterocolitica. Aminoterminal amino
      acid sequencing and mass spectrometry analysis of purified ubiquicidin indicated 
      that it is most likely identical to the ribosomal protein S30. This protein is
      produced by posttranslational processing of the Fau protein, a 133-amino-acid
      fusion protein consisting of S30 linked to an unusual peptide with significant
      homology to ubiquitin. The fau gene has been reported to be expressed in a
      variety of tissues in humans and various animal species. The presence of
      ubiquicidin in the cytosol of macrophages may serve to restrict the intracellular
      growth of microorganisms. In addition, because macrophage disintegration will
      likely lead to release of ubiquicidin into the extracellular environment, it may 
      contribute to host defense after macrophage death.
FAU - Hiemstra, P S
AU  - Hiemstra PS
AD  - Department of Infectious Diseases, Leiden University Medical Center, The
      Netherlands.
FAU - van den Barselaar, M T
AU  - van den Barselaar MT
FAU - Roest, M
AU  - Roest M
FAU - Nibbering, P H
AU  - Nibbering PH
FAU - van Furth, R
AU  - van Furth R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Defensins)
RN  - 0 (Fau protein, mouse)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proteins)
RN  - 0 (Ribosomal Proteins)
RN  - 0 (ribosomal protein S30)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Bacteria/*drug effects
MH  - Cytosol/chemistry
MH  - Defensins
MH  - Escherichia coli/drug effects
MH  - Interferon-gamma/pharmacology
MH  - Listeria monocytogenes/drug effects
MH  - Macrophage Activation/drug effects
MH  - Macrophages/*chemistry/drug effects
MH  - Mass Spectrometry
MH  - Mice
MH  - Microbial Sensitivity Tests
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Neoplasm Proteins/isolation & purification/metabolism
MH  - Proteins/pharmacology
MH  - Rabbits
MH  - Rats
MH  - Ribosomal Proteins/chemistry/metabolism
MH  - Salmonella typhimurium/drug effects
MH  - Staphylococcus aureus/drug effects
MH  - Tumor Cells, Cultured
MH  - Yersinia enterocolitica/drug effects
EDAT- 1999/09/25 00:00
MHDA- 1999/09/25 00:01
CRDT- 1999/09/25 00:00
PHST- 1999/09/25 00:00 [pubmed]
PHST- 1999/09/25 00:01 [medline]
PHST- 1999/09/25 00:00 [entrez]
AID - 10.1002/jlb.66.3.423 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1999 Sep;66(3):423-8. doi: 10.1002/jlb.66.3.423.