PMID- 10495887
OWN - NLM
STAT- MEDLINE
DCOM- 19991215
LR  - 20190905
IS  - 0006-2928 (Print)
IS  - 0006-2928 (Linking)
VI  - 37
IP  - 3-4
DP  - 1999 Apr
TI  - The mouse Rhl1 and Rhag genes: sequence, organization, expression, and
      chromosomal mapping.
PG  - 119-38
AB  - To seek an alternative model for studies of the Rh protein complex, we isolated
      by homology cloning and characterized the mouse Rhced and Rhag genes, which are
      homologous to the human RH and RHAG genes, respectively. Rhced encodes a
      glycoprotein of 418 amino acids which occurs as a composite of human RhD and RhCE
      with 60% identity and 74% similarity. Rhag encodes a glycoprotein of 438 amino
      acids that shares 79% identity and 87% similarity to human Rh50. However, Rhag
      has an elongated C terminus and four N-glycosylation sites clustered on exoloop
      1. Hydropathy plots suggest that Rhl1 and Rhag each span the lipid bilayer 12
      times, with N and C termini facing the cytoplasm. Rhced and Rhag are both
      specified by 10 exons and bear a similar exon/intron structure, but their major
      transcription start sites are mapped at -17A and -27A. Northern analysis revealed
      coexpression of Rhced and Rhag from 11-day embryos throughout adult life in
      erythroid tissues. Southern blotting and linkage analysis showed that Rhced and
      Rhag are single-copy genes localized to chromosomes 4 and 17, respectively; they 
      are paralogous to one another but orthologous to human RH and RHAG. The results
      together predate the occurrence and signify a conserved function of the
      erythroid-specific Rh membrane structures.
FAU - Liu, Z
AU  - Liu Z
AD  - Laboratory of Biochemistry and Molecular Genetics, Lindsley F. Kimball Research
      Institute, New York Blood Center, New York 10021, USA.
FAU - Huang, C H
AU  - Huang CH
LA  - eng
SI  - GENBANK/AF057524
SI  - GENBANK/AF057525
SI  - GENBANK/AF057526
SI  - GENBANK/AF057527
GR  - HL54459/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Genet
JT  - Biochemical genetics
JID - 0126611
RN  - 0 (Blood Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RHAG protein, human)
RN  - 0 (Rhag protein, mouse)
RN  - 0 (Rhced protein, mouse)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - *Blood Proteins
MH  - Blotting, Northern
MH  - *Chromosome Mapping
MH  - DNA, Complementary
MH  - Exons
MH  - Humans
MH  - Introns
MH  - Membrane Glycoproteins/*genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic
MH  - Sequence Homology, Amino Acid
MH  - Transcription, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1999/09/25 00:00
MHDA- 1999/09/25 00:01
CRDT- 1999/09/25 00:00
PHST- 1999/09/25 00:00 [pubmed]
PHST- 1999/09/25 00:01 [medline]
PHST- 1999/09/25 00:00 [entrez]
AID - 10.1023/a:1018726303397 [doi]
PST - ppublish
SO  - Biochem Genet. 1999 Apr;37(3-4):119-38. doi: 10.1023/a:1018726303397.