PMID- 10495276
OWN - NLM
STAT- MEDLINE
DCOM- 20000316
LR  - 20190910
IS  - 0925-4773 (Print)
IS  - 0925-4773 (Linking)
VI  - 87
IP  - 1-2
DP  - 1999 Sep
TI  - Eph receptors and ephrins demarcate cerebellar lobules before and during their
      formation.
PG  - 119-28
AB  - The formation of the ten cerebellar lobules is an unsolved problem in brain
      development. We report a screen for the four subfamilies of Eph receptors and
      their ligands (ephrins) in developing mouse cerebellum, using soluble
      receptor-immunoglobulin and ligand-immunoglobulin fusion proteins, and antibodies
      against EphA and ephrin-B proteins. Our results identify Eph receptors and
      ephrins as the first molecules known to demarcate individual lobules during
      development. Staining for ephrin-A ligands is in lobule VIII as it forms, across 
      the whole width of the cerebellum. Staining for three EphA receptors
      approximately coincides with presumptive lobules VI and/or VII before and just
      after birth, whereas a fourth EphA receptor (EphA4, which binds ligands of both
      subfamilies) has more widespread expression. Staining for EphB receptors is in
      lobules VII, VIII, and IX. Staining for ephrin-B ligands is much weaker, becomes 
      detectable only after birth, and does not appear to be lobule-specific. Staining 
      for all subfamilies spreads to at least some adjacent lobules as maturation
      proceeds. The lobule-specific patterns appear before the lobules form, and
      initially extend across the width of the cerebellum, in spite of the lesser
      conservation of the lateral extensions of the lobules. These expression patterns 
      define previously unknown developmental units and suggest that Eph family
      proteins may contribute to cerebellar morphogenesis.
FAU - Rogers, J H
AU  - Rogers JH
AD  - Department of Physiology, University of Cambridge, Downing St., Cambridge, UK.
      jr@mole.bio.cam.ac.uk
FAU - Ciossek, T
AU  - Ciossek T
FAU - Menzel, P
AU  - Menzel P
FAU - Pasquale, E B
AU  - Pasquale EB
LA  - eng
GR  - HD25938/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Mech Dev
JT  - Mechanisms of development
JID - 9101218
RN  - 0 (Ephrin-A2)
RN  - 0 (Ephrin-A5)
RN  - 0 (Ephrin-B1)
RN  - 0 (Epitopes)
RN  - 0 (Fetal Proteins)
RN  - 0 (Immunoglobulin Fc Fragments)
RN  - 0 (Membrane Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, EphA4)
RN  - EC 2.7.10.1 (Receptor, EphA7)
SB  - IM
MH  - Animals
MH  - Cerebellum/*embryology/*metabolism
MH  - Ephrin-A2
MH  - Ephrin-A5
MH  - Ephrin-B1
MH  - Epitopes/metabolism
MH  - Fetal Proteins/metabolism
MH  - Immunoglobulin Fc Fragments/metabolism
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Membrane Proteins/*metabolism
MH  - Mice
MH  - Receptor Protein-Tyrosine Kinases/*metabolism
MH  - Receptor, EphA4
MH  - Receptor, EphA7
MH  - Time Factors
MH  - Transcription Factors/metabolism
EDAT- 1999/09/24 09:00
MHDA- 2000/03/18 09:00
CRDT- 1999/09/24 09:00
PHST- 1999/09/24 09:00 [pubmed]
PHST- 2000/03/18 09:00 [medline]
PHST- 1999/09/24 09:00 [entrez]
AID - S0925-4773(99)00154-9 [pii]
AID - 10.1016/s0925-4773(99)00154-9 [doi]
PST - ppublish
SO  - Mech Dev. 1999 Sep;87(1-2):119-28. doi: 10.1016/s0925-4773(99)00154-9.