PMID- 10494861
OWN - NLM
STAT- MEDLINE
DCOM- 19991103
LR  - 20151119
IS  - 0171-9335 (Print)
IS  - 0171-9335 (Linking)
VI  - 78
IP  - 8
DP  - 1999 Aug
TI  - The mammalian interphase centrosome: two independent units maintained together by
      the dynamics of the microtubule cytoskeleton.
PG  - 549-60
AB  - In mammalian cells the centrosome or diplosome is defined by the two parental
      centrioles observed in electron microscopy and by the pericentriolar material
      immunostained with several antibodies directed against various centrosomal
      proteins (gamma-tubulin, pericentrin, centrin and centractin). Partial
      destabilization of the microtubule cytoskeleton by microtubule-disassembling
      substances induced a splitting and a slow migration of the two diplosome units to
      opposite nuclear sides during most of the interphase in several mammalian cell
      lines. These units relocated close together following drug removal, while
      microtubule stabilization by nM taxol concentrations inhibited this process.
      Cytochalasin slowed down diplosome splitting but did not affect its relocation
      after colcemid washing. These results account for the apparently opposite effects
      induced by microtubule poisons on centriole separation. Moreover, they provide
      new information concerning the centrosome cycle and stability. First, the
      centrosome is formed by two units, distinguished only by the number of attached
      stable microtubules, but not by pericentrin, gamma-tubulin, centrin and
      centractin and their potency to nucleate microtubules. Second, the centrosomal
      units are independent during most of the interphase. Third, according to the cell
      type, these centrosomal units are localized in close proximity because they are
      either linked or maintained close together by the normal dynamics of the
      microtubule cytoskeleton. Finally, the relocalization of the centrosomal units
      with their centrioles in cells possessing one or two centrosomes suggests that
      their relative position results from the overall tensional forces involving at
      least partially the microtubule arrays nucleated by each of these entities.
FAU - Jean, C
AU  - Jean C
AD  - Institut de Pharmacologie et de Biologie Structurale, CNRS, Toulouse, France.
FAU - Tollon, Y
AU  - Tollon Y
FAU - Raynaud-Messina, B
AU  - Raynaud-Messina B
FAU - Wright, M
AU  - Wright M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Cell Biol
JT  - European journal of cell biology
JID - 7906240
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Tubulin)
RN  - P88XT4IS4D (Paclitaxel)
RN  - Z01IVE25KI (Demecolcine)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents, Phytogenic/pharmacology
MH  - Cell Line/cytology
MH  - Centrosome/drug effects/*physiology/ultrastructure
MH  - Cricetinae
MH  - Cytoskeleton/chemistry/ultrastructure
MH  - Demecolcine/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Interphase/drug effects/*physiology
MH  - Microtubules/*chemistry/ultrastructure
MH  - Paclitaxel/pharmacology
MH  - Rats
MH  - Tubulin/metabolism
MH  - Tumor Cells, Cultured/cytology
MH  - Xenopus
EDAT- 1999/09/24 00:00
MHDA- 1999/09/24 00:01
CRDT- 1999/09/24 00:00
PHST- 1999/09/24 00:00 [pubmed]
PHST- 1999/09/24 00:01 [medline]
PHST- 1999/09/24 00:00 [entrez]
AID - S0171-9335(99)80020-X [pii]
AID - 10.1016/S0171-9335(99)80020-X [doi]
PST - ppublish
SO  - Eur J Cell Biol. 1999 Aug;78(8):549-60. doi: 10.1016/S0171-9335(99)80020-X.