PMID- 10493933
OWN - NLM
STAT- MEDLINE
DCOM- 19991207
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 343 Pt 1
DP  - 1999 Oct 1
TI  - Structure and expression of murine mgcRacGAP: its developmental regulation
      suggests a role for the Rac/MgcRacGAP signalling pathway in neurogenesis.
PG  - 225-30
AB  - Rho-family GTPases regulate a wide range of biological functions including cell
      migration, cell adhesion and cell growth. Recently, results from studies in vivo 
      in Drosophila, mouse and humans have demonstrated the involvement of these
      GTPases in mechanisms controlling neuronal differentiation and the development of
      the central nervous system (CNS). However, the signalling pathways underlying
      these functions and the proteins directly regulating RhoGTPases in developing
      neurons are poorly defined. Here we report the structure and expression pattern
      of the murine orthologue of mgcRacGAP, a human gene encoding a RacGTPase partner 
      expressed in male germ cells [Toure, Dorseuil, Morin, Timmons, Jegou, Reibel and 
      Gacon (1998) J. Biol. Chem. 273, 6019-6023]. In contrast with that from humans,
      murine mgcRacGAP encodes two distinct transcripts. Both are developmentally
      regulated. A 2.2 kb transcript is strongly expressed in mature testis and is
      up-regulated with spermatogenesis. A 3 kb RNA is predominant in the embryo and is
      expressed primarily in the CNS during the neurogenic phase, decreasing after
      birth. In situ hybridization analysis in embryonic-day 14.5 mouse embryos
      demonstrates a preferential expression of mgcRacGAP in the proliferative
      ventricular zone of the cortex. In addition to the expression of mgcRacGAP in
      male germ cells already reported in humans and suggesting an involvement in
      spermatogenesis, we characterize an embryonic transcript whose expression is
      closely correlated with neurogenesis. This result addresses the question of the
      role of Rac/MgcRacGAP pathway in neuronal proliferation.
FAU - Arar, C
AU  - Arar C
AD  - Institut Cochin de Genetique Moleculaire, INSERM Unite 257, 24 Rue du Faubourg
      Saint Jacques, 75014 Paris, France.
FAU - Ott, M O
AU  - Ott MO
FAU - Toure, A
AU  - Toure A
FAU - Gacon, G
AU  - Gacon G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (DNA, Complementary)
RN  - 0 (GTPase-Activating Proteins)
RN  - 0 (mgcRacGAP)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain/*embryology
MH  - DNA, Complementary
MH  - GTPase-Activating Proteins/chemistry/genetics/*metabolism
MH  - *Gene Expression Regulation, Developmental
MH  - Humans
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Sequence Homology, Amino Acid
MH  - *Signal Transduction
MH  - Testis/embryology
PMC - PMC1220545
EDAT- 1999/09/24 00:00
MHDA- 1999/09/24 00:01
CRDT- 1999/09/24 00:00
PHST- 1999/09/24 00:00 [pubmed]
PHST- 1999/09/24 00:01 [medline]
PHST- 1999/09/24 00:00 [entrez]
PST - ppublish
SO  - Biochem J. 1999 Oct 1;343 Pt 1:225-30.