PMID- 10493931
OWN - NLM
STAT- MEDLINE
DCOM- 19991207
LR  - 20220227
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 343 Pt 1
DP  - 1999 Oct 1
TI  - Identification of two human dimethylarginine dimethylaminohydrolases with
      distinct tissue distributions and homology with microbial arginine deiminases.
PG  - 209-14
AB  - Methylarginines inhibit nitric oxide synthases (NOS). Cellular concentrations of 
      methylarginines are determined in part by the activity of dimethylarginine
      dimethylaminohydrolase (DDAH; EC 3.5.3. 18). We have cloned human DDAH and
      identified and expressed a second novel DDAH isoform (DDAH I and II
      respectively). DDAH I predominates in tissues that express neuronal NOS. DDAH II 
      predominates in tissues expressing endothelial NOS. These results strengthen the 
      hypothesis that methylarginine concentration is actively regulated and identify
      molecular targets for the tissue and cell-specific regulation of methylarginine
      concentration.
FAU - Leiper, J M
AU  - Leiper JM
AD  - Centre for Clinical Pharmacology, The Rayne Institute, University College London,
      5 University Street, London, U.K.
FAU - Santa Maria, J
AU  - Santa Maria J
FAU - Chubb, A
AU  - Chubb A
FAU - MacAllister, R J
AU  - MacAllister RJ
FAU - Charles, I G
AU  - Charles IG
FAU - Whitley, G S
AU  - Whitley GS
FAU - Vallance, P
AU  - Vallance P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (DNA, Complementary)
RN  - 0 (Isoenzymes)
RN  - 0 (Recombinant Proteins)
RN  - EC 3.- (Hydrolases)
RN  - EC 3.5.- (Amidohydrolases)
RN  - EC 3.5.3.18 (dimethylargininase)
SB  - IM
MH  - *Amidohydrolases
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Humans
MH  - Hydrolases/chemistry/genetics/*metabolism
MH  - Isoenzymes/chemistry/genetics/*metabolism
MH  - Molecular Sequence Data
MH  - Rats
MH  - Recombinant Proteins/chemistry/genetics/metabolism
MH  - Sequence Homology, Amino Acid
PMC - PMC1220543
EDAT- 1999/09/24 00:00
MHDA- 1999/09/24 00:01
CRDT- 1999/09/24 00:00
PHST- 1999/09/24 00:00 [pubmed]
PHST- 1999/09/24 00:01 [medline]
PHST- 1999/09/24 00:00 [entrez]
PST - ppublish
SO  - Biochem J. 1999 Oct 1;343 Pt 1:209-14.