PMID- 10493891
OWN - NLM
STAT- MEDLINE
DCOM- 19991021
LR  - 20131121
IS  - 0014-4800 (Print)
IS  - 0014-4800 (Linking)
VI  - 67
IP  - 1
DP  - 1999 Sep
TI  - Fibrosin: A novel lymphokine in alcohol-induced fibrosis.
PG  - 40-9
AB  - In this study, we examined the role of fibrogenic cytokines in alcohol-induced
      fibrosis. In particular, we examined the production of a novel fibrogenic
      cytokine, fibrosin, among others, by fibroblasts in response to ethanol in vitro;
      we also studied the production of fibrosin in an animal model of alcohol-induced 
      liver injury. This model system utilizes the intragastric feeding rat model in
      which rats are fed different dietary fats and ethanol or dextrose. Our study
      showed that physiologic concentrations of ethanol directly induced proliferation 
      of fibroblasts in vitro and also stimulated the production of cytokines. In
      particular, fibrosin, the novel fibrogenic cytokine, was produced. Other
      cytokines such as TGFbeta, IL-6, and TNFalpha were also induced. Also, exposure
      of fibroblasts to interleukin-1beta, interleukin-6, and tumor necrosis factor
      alpha induced production of fibrosin. In the fish oil-ethanol-fed rats which
      showed fibrotic lesions in the liver, fibrosin mRNA as well as protein was
      expressed. Fibrosin was not detected in control rats not exhibiting fibrosis.
      These studies show that ethanol can directly stimulate fibroblast proliferation
      and production of fibrogenic cytokines. It is likely that fibrosin, which may be 
      derived from inflammatory cells, contributes to alcohol-induced hepatic fibrosis 
      in vivo.
CI  - Copyright 1999 Academic Press.
FAU - Prakash, S
AU  - Prakash S
AD  - Center for Cancer Research, Massachusetts Institute of Technology, Cambridge
      02139, Massachusetts.
FAU - Nanji, A A
AU  - Nanji AA
FAU - Robbins, P W
AU  - Robbins PW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
RN  - 0 (Cytokines)
RN  - 0 (DNA Primers)
RN  - 0 (Lymphokines)
RN  - 0 (RNA, Messenger)
RN  - 0 (fibrosin)
RN  - 3K9958V90M (Ethanol)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Collagen/analysis
MH  - Cytokines/biosynthesis/pharmacology
MH  - DNA Primers/chemistry
MH  - Dose-Response Relationship, Drug
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Ethanol/pharmacology
MH  - Fibroblasts/metabolism
MH  - Humans
MH  - Liver/drug effects/*metabolism/pathology
MH  - Liver Cirrhosis, Alcoholic/etiology/*metabolism/pathology
MH  - Lymphokines/*biosynthesis/genetics
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 1999/09/24 00:00
MHDA- 1999/09/24 00:01
CRDT- 1999/09/24 00:00
PHST- 1999/09/24 00:00 [pubmed]
PHST- 1999/09/24 00:01 [medline]
PHST- 1999/09/24 00:00 [entrez]
AID - 10.1006/exmp.1999.2274 [doi]
AID - S0014-4800(99)92274-8 [pii]
PST - ppublish
SO  - Exp Mol Pathol. 1999 Sep;67(1):40-9. doi: 10.1006/exmp.1999.2274.