PMID- 10493836
OWN - NLM
STAT- MEDLINE
DCOM- 19991122
LR  - 20071114
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 60
IP  - 3
DP  - 1999 Sep 15
TI  - Cloning of the human monocarboxylate transporter MCT3 gene: localization to
      chromosome 22q12.3-q13.2.
PG  - 366-70
AB  - Lactate transport across cell membranes is mediated by a family of proton-coupled
      monocarboxylate transporters (MCTs). The retinal pigment epithelium (RPE)
      expresses a unique member of this family, MCT3. A portion of the human MCT3 gene 
      was cloned by polymerase chain reaction using primers designed from rat RPE MCT3 
      cDNA sequence. The human genomic sequence was used to design primers to clone
      human MCT3 cDNA and to identify a bacterial artificial chromosome clone
      containing the human MCT3 gene. The human MCT3 cDNA contained a 1512-nucleotide
      open reading frame with a deduced amino sequence 85% identical to rat MCT3.
      Comparison of the cDNA and genomic sequences revealed that the MCT3 gene was
      composed of five exons distributed over 5 kb of DNA. The exon-intron borders were
      conserved between the human and the chicken MCT3 genes. Using radiation hybrid
      mapping, the MCT3 gene was mapped to chromosome 22 between markers WI11639 and
      SGC30687. A search of chromosome 22 in the Sanger Centre database confirmed the
      location of the human MCT3 gene at 22q12.3-q13.2.
CI  - Copyright 1999 Academic Press.
FAU - Yoon, H
AU  - Yoon H
AD  - Laboratory for Cell and Molecular Biology, Pennsylvania College of Optometry,
      8360 Old York Road, Elkins Park, Pennsylvania 19027-1598, USA.
FAU - Donoso, L A
AU  - Donoso LA
FAU - Philp, N J
AU  - Philp NJ
LA  - eng
SI  - GENBANK/AF132610
SI  - GENBANK/AF132611
GR  - EY12042/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Mct3 protein, rat)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Monocarboxylic Acid Transporters)
RN  - 0 (Protein Isoforms)
RN  - 0 (SLC16A3 protein, human)
SB  - IM
MH  - Animals
MH  - Carrier Proteins/*genetics
MH  - Chickens
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 22/*genetics
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry
MH  - Humans
MH  - *Membrane Transport Proteins
MH  - Molecular Sequence Data
MH  - Monocarboxylic Acid Transporters
MH  - Protein Isoforms/genetics
MH  - Rats
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Nucleic Acid
EDAT- 1999/09/24 00:00
MHDA- 1999/09/24 00:01
CRDT- 1999/09/24 00:00
PHST- 1999/09/24 00:00 [pubmed]
PHST- 1999/09/24 00:01 [medline]
PHST- 1999/09/24 00:00 [entrez]
AID - 10.1006/geno.1999.5926 [doi]
AID - S0888-7543(99)95926-8 [pii]
PST - ppublish
SO  - Genomics. 1999 Sep 15;60(3):366-70. doi: 10.1006/geno.1999.5926.