PMID- 10492399
OWN - NLM
STAT- MEDLINE
DCOM- 19990930
LR  - 20081121
IS  - 1044-5498 (Print)
IS  - 1044-5498 (Linking)
VI  - 18
IP  - 9
DP  - 1999 Sep
TI  - Cloning and functional analysis of the human IRF-3 promoter.
PG  - 685-92
AB  - We have isolated a genomic clone of the human IRF-3 gene containing 779
      nucleotides of the 5' flanking region and the complete intron exon sequence. The 
      gene has eight exons which span about 6 kb on chromosome 19q13.3. The IRF-3
      promoter has neither a conserved TATA box nor a CCAAT box motif but is GC rich.
      Several putative DNA-binding elements were identified, including three SP-1
      sites, a USF element, a HOX box, a CarG box, and an NF-1 site. Deletion analysis 
      of the promoter region showed that the core basal promoter, consisting of 113 bp 
      5' of the first transcription start site, was sufficient for constitutive
      expression. This region contains only one of the SP-1 sites as well as the HOX
      element and NF-1 site, and although it is GC rich, it does not contain any of the
      other putative DNA-binding sites. In contrast, the mouse IRF-3 promoter, while
      displaying a high degree of homology with the human promoter, contains both TATA 
      and CCAAT box motifs, suggesting that, at least at the level of transcription
      initiation, these genes may be differentially regulated.
FAU - Lowther, W J
AU  - Lowther WJ
AD  - Oncology Center, School of Medicine, The Johns Hopkins University, Baltimore,
      Maryland 21231, USA.
FAU - Moore, P A
AU  - Moore PA
FAU - Carter, K C
AU  - Carter KC
FAU - Pitha, P M
AU  - Pitha PM
LA  - eng
SI  - GENBANK/AF112181
PT  - Journal Article
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (IRF3 protein, human)
RN  - 0 (Interferon Regulatory Factor-3)
RN  - 0 (Irf3 protein, mouse)
RN  - 0 (Transcription Factors)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 19
MH  - Cloning, Molecular
MH  - DNA
MH  - DNA-Binding Proteins/*genetics
MH  - Exons
MH  - Humans
MH  - Interferon Regulatory Factor-3
MH  - Introns
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Promoter Regions, Genetic
MH  - Transcription Factors/*genetics
EDAT- 1999/09/24 00:00
MHDA- 1999/09/24 00:01
CRDT- 1999/09/24 00:00
PHST- 1999/09/24 00:00 [pubmed]
PHST- 1999/09/24 00:01 [medline]
PHST- 1999/09/24 00:00 [entrez]
AID - 10.1089/104454999314962 [doi]
PST - ppublish
SO  - DNA Cell Biol. 1999 Sep;18(9):685-92. doi: 10.1089/104454999314962.