PMID- 10492170
OWN - NLM
STAT- MEDLINE
DCOM- 19991014
LR  - 20190513
IS  - 1340-2838 (Print)
IS  - 1340-2838 (Linking)
VI  - 6
IP  - 4
DP  - 1999 Aug 31
TI  - Sequence analysis of Stx2-converting phage VT2-Sa shows a great divergence in
      early regulation and replication regions.
PG  - 235-40
AB  - In enterohemorrhagic Escherichia coli, Shiga toxin is produced by lysogenic
      prophages. We have isolated the prophage VT2-Sa that is responsible for
      production of Shiga toxin type 2 protein, and determined the complete nucleotide 
      sequence of this phage DNA. The entire DNA sequence consisted of 60,942 bp,
      exhibiting marked similarity to the 933W phage genome. However, several
      differences were observed in the immunity and replication regions, where cI, cII,
      cIII, N, cro, O, and P genes were present: Predicted amino acid sequences of N,
      cI, cro, O and P in the VT2-Sa genome did not show significant similarity to the 
      counterparts of the 933W genome; however its cI showed higher similarity to
      lambda. Furthermore, O and P closely resembled those of phage HK022. These
      observations suggest that the various degrees of homology observed in the
      immunity and replication regions of VT2-Sa could have resulted from frequent
      recombination events among the lambdoid phages, and that these regions play a key
      role as a functional unit for phage propagation in competition with other
      lambdoid phages.
FAU - Miyamoto, H
AU  - Miyamoto H
AD  - Faculty of Biology-Oriented Science and Technology, Department of Molecular
      Genetics, Kinki University, Uchita, Wakayama, Japan.
      miyamoto@gene.waka.kindai.ac.jp
FAU - Nakai, W
AU  - Nakai W
FAU - Yajima, N
AU  - Yajima N
FAU - Fujibayashi, A
AU  - Fujibayashi A
FAU - Higuchi, T
AU  - Higuchi T
FAU - Sato, K
AU  - Sato K
FAU - Matsushiro, A
AU  - Matsushiro A
LA  - eng
SI  - GENBANK/AP000363
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - DNA Res
JT  - DNA research : an international journal for rapid publication of reports on genes
      and genomes
JID - 9423827
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
RN  - 0 (Shiga Toxins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Bacterial Toxins/*genetics
MH  - Bacteriophage lambda/genetics
MH  - Base Sequence
MH  - Coliphages/*genetics
MH  - Enterotoxins/*genetics
MH  - Escherichia coli O157/*virology
MH  - Gene Expression Regulation
MH  - Genes, Immediate-Early
MH  - Genes, Viral
MH  - Humans
MH  - Molecular Sequence Data
MH  - Sequence Homology, Amino Acid
MH  - Shiga Toxins
MH  - *Shigella dysenteriae
EDAT- 1999/09/24 00:00
MHDA- 1999/09/24 00:01
CRDT- 1999/09/24 00:00
PHST- 1999/09/24 00:00 [pubmed]
PHST- 1999/09/24 00:01 [medline]
PHST- 1999/09/24 00:00 [entrez]
AID - 10.1093/dnares/6.4.235 [doi]
PST - ppublish
SO  - DNA Res. 1999 Aug 31;6(4):235-40. doi: 10.1093/dnares/6.4.235.