PMID- 10491181
OWN - NLM
STAT- MEDLINE
DCOM- 19991122
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 265
IP  - 1
DP  - 1999 Oct 1
TI  - Stage-specific expression of alpha1,2-fucosyltransferase and alpha1,
      3-fucosyltransferase (FT) during mouse embryogenesis.
PG  - 258-63
AB  - Lex [Galbeta1-4(Fucalpha1-3)GlcNAc] and Ley
      [Fucalpha1-2Galbeta1-4(Fucalpha1-3)GlcNAc] are both stage-specific embryonic
      antigens. Lex is first detected on the blastomeres of the 8-cell stage embryo,
      which correlates with the onset of blastomere compaction. Ley is highly expressed
      on the surface of the blastocyst, which has been shown to be involved in
      blastocyst attachment in the mouse. In the present study, mouse alpha1,2-FT (also
      known as FUT1) and alpha1,3-FT (also known as Fuc-TIV), which were responsible
      for Lex and Ley formation, were examined in preimplantation stage embryos by
      reverse transcription-PCR and in situ hybridization. alpha1,3-FT mRNA was
      detected in all embryos of preimplantation stage, while alpha1,2-FT mRNA emerged 
      in the later stage embryos from 8-cell to 16-cell to the blastocyst. These
      results indicated the expression of Ley was regulated by alpha1,2-FT. In situ
      hybridization showed that these two enzyme mRNAs were detected only in morula and
      blastocyst stage embryos. The alpha1,2-FT and alpha1, 3-FT mRNAs were located in 
      both the inner cell mass and the trophoblast cells. 2-Cell and 4-cell embryos
      were isolated from the oviduct and cultured in vitro to the 8-cell, morula and
      blastocyst stage. The expression of alpha1,2-FT and alpha1,3-FT were observed in 
      these embryos developed in vitro; immunohistochemical analysis also showed that
      Ley expression was positive. These results suggested the stage-specific
      expression of Ley on the embryos was synthesized by endogenous alpha1,2-FT and
      alpha1,3-FT rather than transfer from other sources. In addition, the expression 
      of alpha1, 2-FT was differentially regulated and the uterine factor was not
      prerequisite of the expression of Ley.
FAU - Liu, N
AU  - Liu N
AD  - Department of Biochemistry, Dalian Medical University, China.
FAU - Jin, C
AU  - Jin C
FAU - Zhu, Z M
AU  - Zhu ZM
FAU - Zhang, J
AU  - Zhang J
FAU - Tao, H
AU  - Tao H
FAU - Ge, C
AU  - Ge C
FAU - Yang, S
AU  - Yang S
FAU - Zhang, S
AU  - Zhang S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Lewis Blood-Group System)
RN  - 0 (Lewis X Antigen)
RN  - 0 (Lewis Y antigen)
RN  - EC 2.4.1.- (Fucosyltransferases)
RN  - EC 2.4.1.152 (galactoside 3-fucosyltransferase)
RN  - EC 2.4.1.69 (galactoside 2-alpha-L-fucosyltransferase)
SB  - IM
MH  - Animals
MH  - Blastocyst/*enzymology
MH  - Fucosyltransferases/genetics/*isolation & purification
MH  - Gene Expression
MH  - In Situ Hybridization
MH  - In Vitro Techniques
MH  - *Lewis Blood-Group System/*biosynthesis
MH  - Lewis X Antigen/*biosynthesis
MH  - Mice
MH  - Tissue Distribution
EDAT- 1999/09/22 00:00
MHDA- 1999/09/22 00:01
CRDT- 1999/09/22 00:00
PHST- 1999/09/22 00:00 [pubmed]
PHST- 1999/09/22 00:01 [medline]
PHST- 1999/09/22 00:00 [entrez]
AID - ejb728 [pii]
AID - 10.1046/j.1432-1327.1999.00728.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Oct 1;265(1):258-63. doi: 10.1046/j.1432-1327.1999.00728.x.