PMID- 10491017
OWN - NLM
STAT- MEDLINE
DCOM- 19991216
LR  - 20181113
IS  - 1219-4956 (Print)
IS  - 1219-4956 (Linking)
VI  - 5
IP  - 3
DP  - 1999
TI  - Identification and characterization of different subpopulations in a human lung
      adenocarcinoma cell line (A549).
PG  - 197-204
AB  - The morphology, cell growth, antigenic expression and tumorigenicity of cell
      subpopulations from the A549 lung adenocarcinoma isolated by Percoll gradient
      separation have been analysed. Four subpopulations were obtained (subpopulations 
      A, B, C and D). Immunocytochemical analysis of several antigens was performed
      with monoclonal antibodies (MAbs): MUC1 mucin (C595, HMFG1 and HMFG2), MUC5B
      (PANH2); gp230 (PANH4); carbohydrate antigens including sialyl Lewis x (KM93), Tn
      antigen (83D4), Lewis y (C14); 5, 6, 8, 17 and 19 cytokeratins and p53. The cell 
      population D tended to form cell aggregates that piled up on the monolayer
      similar to overgrowth cultures of the A549 parental cell line, whereas A, B and C
      cell subpopulations formed well spread monolayers. Both parental A549 and
      subpopulation D secreted abundant mucus. The topographic distribution and
      secretion production were correlated with tumorigenic assays since only
      subpopulation D grew in nude mice exhibiting reduced latency period; these
      characteristics correlated with the fast growth of the subpopulation D in vitro. 
      Immunocytochemical analysis demonstrated that subpopulation D showed greater
      expression of MUC1 mucin and carbohydrate antigens such as Tn antigen, sialyl
      Lewis x and Lewis y and less expression of cytokeratins, p53, MUC5B and gp230;
      conversely, subpopulations A, B and C showed the opposite antigenic profile. Our 
      results illustrate heterogeneity in the A549 cell line; subpopulations A, B and C
      retained characteristics of more differentiated adenocarcinoma while
      subpopulation D displayed features of a less differentiated tumor line.
FAU - Croce, M V
AU  - Croce MV
AD  - Universidad Nacional de la Plata, Centro de Investigaciones Inmunologicas Basicas
      y Aplicadas (CINIBA) Calle 60 y 120, La Plata, Argentina.
FAU - Colussi, A G
AU  - Colussi AG
FAU - Price, M R
AU  - Price MR
FAU - Segal-Eiras, A
AU  - Segal-Eiras A
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Pathol Oncol Res
JT  - Pathology oncology research : POR
JID - 9706087
RN  - 0
      (5-acetylneuraminyl-(2-3)-galactosyl-(1-4)-(fucopyranosyl-(1-3))-N-acetylglucosam
      ine)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Oligosaccharides)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Adenocarcinoma/immunology/*pathology
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Cell Separation
MH  - Humans
MH  - Immunohistochemistry
MH  - Keratins/analysis
MH  - Lung Neoplasms/immunology/*pathology
MH  - Mice
MH  - Mice, Nude
MH  - Neoplasm Transplantation
MH  - Oligosaccharides/analysis
MH  - Phenotype
MH  - Time Factors
MH  - Tumor Cells, Cultured
MH  - Tumor Suppressor Protein p53/analysis
EDAT- 1999/09/22 00:00
MHDA- 1999/09/22 00:01
CRDT- 1999/09/22 00:00
PHST- 1999/09/22 00:00 [pubmed]
PHST- 1999/09/22 00:01 [medline]
PHST- 1999/09/22 00:00 [entrez]
AID - PAOR.1999.5.3.0197 [pii]
PST - ppublish
SO  - Pathol Oncol Res. 1999;5(3):197-204.