PMID- 10490106
OWN - NLM
STAT- MEDLINE
DCOM- 19991001
LR  - 20220318
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 98
IP  - 5
DP  - 1999 Sep 3
TI  - Regulation of hormone-induced histone hyperacetylation and gene activation via
      acetylation of an acetylase.
PG  - 675-86
AB  - Nuclear receptors have been postulated to regulate gene expression via their
      association with histone acetylase (HAT) or deacetylase complexes. We report that
      hormone induces dramatic hyperacetylation at endogenous target genes through the 
      HAT activity of p300/CBP. Unexpectedly, this hyperacetylation is transient and
      coincides with attenuation of hormone-induced gene activation. In exploring the
      underlying mechanism, we found that the acetylase ACTR can be acetylated by
      p300/CBP. The acetylation neutralizes the positive charges of two lysine residues
      adjacent to the core LXXLL motif and disrupts the association of HAT coactivator 
      complexes with promoter-bound estrogen receptors. These results provide strong in
      vivo evidence that histone acetylation plays a key role in hormone-induced gene
      activation and define cofactor acetylation as a novel regulatory mechanism in
      hormonal signaling.
FAU - Chen, H
AU  - Chen H
AD  - The Salk Institute for Biological Studies, La Jolla, California 92037, USA.
FAU - Lin, R J
AU  - Lin RJ
FAU - Xie, W
AU  - Xie W
FAU - Wilpitz, D
AU  - Wilpitz D
FAU - Evans, R M
AU  - Evans RM
LA  - eng
GR  - GM26444/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Histones)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Trans-Activators)
RN  - EC 2.3.1.- (Acetyltransferases)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
SB  - IM
MH  - Acetylation
MH  - Acetyltransferases/*metabolism
MH  - *Gene Expression Regulation
MH  - HL-60 Cells
MH  - Histone Acetyltransferases
MH  - Histones/agonists/*metabolism
MH  - Humans
MH  - Kinetics
MH  - Models, Genetic
MH  - Models, Molecular
MH  - Mutagenesis
MH  - Nuclear Proteins/metabolism
MH  - Receptors, Cytoplasmic and Nuclear/genetics/metabolism
MH  - Recombinant Proteins/metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Signal Transduction
MH  - Time Factors
MH  - Trans-Activators/metabolism
MH  - Transcriptional Activation
EDAT- 1999/09/18 00:00
MHDA- 1999/09/18 00:01
CRDT- 1999/09/18 00:00
PHST- 1999/09/18 00:00 [pubmed]
PHST- 1999/09/18 00:01 [medline]
PHST- 1999/09/18 00:00 [entrez]
AID - S0092-8674(00)80054-9 [pii]
AID - 10.1016/s0092-8674(00)80054-9 [doi]
PST - ppublish
SO  - Cell. 1999 Sep 3;98(5):675-86. doi: 10.1016/s0092-8674(00)80054-9.