PMID- 10490026
OWN - NLM
STAT- MEDLINE
DCOM- 19990927
LR  - 20061115
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 401
IP  - 6749
DP  - 1999 Sep 9
TI  - Acinus is a caspase-3-activated protein required for apoptotic chromatin
      condensation.
PG  - 168-73
AB  - Apoptosis is defined by several unique morphological nuclear changes, such as
      chromatin condensation and nuclear fragmentation. These changes are triggered by 
      the activation of a family of cysteine proteases called caspases, and
      caspase-activated DNase (CAD/DFF40) and lamin protease (caspase-6) have been
      implicated in some of these changes. CAD/DFF40 induces chromatin condensation in 
      purified nuclei, but distinct caspase-activated factor(s) may be responsible for 
      chromatin condensation. Here we use an in vitro system to identify a new nuclear 
      factor, designated Acinus, which induces apoptotic chromatin condensation after
      cleavage by caspase-3 without inducing DNA fragmentation. Immunodepletion
      experiments showed that Acinus is essential for apoptotic chromatin condensation 
      in vitro, and an antisense study revealed that Acinus is also important in the
      induction of apoptotic chromatin condensation in cells.
FAU - Sahara, S
AU  - Sahara S
AD  - Department of Medical Genetics, Biomedical Research Center, Osaka University
      Medical School, Suita, Japan.
FAU - Aoto, M
AU  - Aoto M
FAU - Eguchi, Y
AU  - Eguchi Y
FAU - Imamoto, N
AU  - Imamoto N
FAU - Yoneda, Y
AU  - Yoneda Y
FAU - Tsujimoto, Y
AU  - Tsujimoto Y
LA  - eng
SI  - GENBANK/AF124725
SI  - GENBANK/AF124726
SI  - GENBANK/AF124727
SI  - GENBANK/AF124728
SI  - GENBANK/AF124729
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (ACIN1 protein, human)
RN  - 0 (Chromatin)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Proteins)
RN  - EC 3.4.22.- (CASP3 protein, human)
RN  - EC 3.4.22.- (Casp3 protein, mouse)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
CIN - Nature. 1999 Sep 9;401(6749):127-8. PMID: 10490018
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Apoptosis
MH  - Biological Transport
MH  - COS Cells
MH  - Caspase 3
MH  - Caspases/*metabolism
MH  - Cattle
MH  - Cell Nucleus/physiology
MH  - Chromatin/*physiology
MH  - Cloning, Molecular
MH  - DNA Fragmentation
MH  - HeLa Cells
MH  - Humans
MH  - Jurkat Cells
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/genetics/isolation & purification/*physiology
MH  - Recombinant Proteins/genetics/pharmacology
MH  - Sequence Homology, Amino Acid
MH  - Thymus Gland/metabolism
EDAT- 1999/09/18 09:00
MHDA- 2001/03/23 10:01
CRDT- 1999/09/18 09:00
PHST- 1999/09/18 09:00 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/09/18 09:00 [entrez]
AID - 10.1038/43678 [doi]
PST - ppublish
SO  - Nature. 1999 Sep 9;401(6749):168-73. doi: 10.1038/43678.