PMID- 10489376
OWN - NLM
STAT- MEDLINE
DCOM- 19991007
LR  - 20190619
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 285
IP  - 5435
DP  - 1999 Sep 17
TI  - Acute activation of Maxi-K channels (hSlo) by estradiol binding to the beta
      subunit.
PG  - 1929-31
AB  - Maxi-K channels consist of a pore-forming alpha subunit and a regulatory beta
      subunit, which confers the channel with a higher Ca(2+) sensitivity. Estradiol
      bound to the beta subunit and activated the Maxi-K channel (hSlo) only when both 
      alpha and beta subunits were present. This activation was independent of the
      generation of intracellular signals and could be triggered by estradiol
      conjugated to a membrane-impenetrable carrier protein. This study documents the
      direct interaction of a hormone with a voltage-gated channel subunit and provides
      the molecular mechanism for the modulation of vascular smooth muscle Maxi-K
      channels by estrogens.
FAU - Valverde, M A
AU  - Valverde MA
AD  - Departament de Ciencies Experimentals i de la Salut, Universidad Pompeu Fabra,
      C/Doctor Aiguader 80, 08003 Barcelona, Spain. miguel.valverde@cexs.upf.es
FAU - Rojas, P
AU  - Rojas P
FAU - Amigo, J
AU  - Amigo J
FAU - Cosmelli, D
AU  - Cosmelli D
FAU - Orio, P
AU  - Orio P
FAU - Bahamonde, M I
AU  - Bahamonde MI
FAU - Mann, G E
AU  - Mann GE
FAU - Vergara, C
AU  - Vergara C
FAU - Latorre, R
AU  - Latorre R
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (KCNMA1 protein, human)
RN  - 0 (Large-Conductance Calcium-Activated Potassium Channel alpha Subunits)
RN  - 0 (Large-Conductance Calcium-Activated Potassium Channel beta Subunits)
RN  - 0 (Large-Conductance Calcium-Activated Potassium Channels)
RN  - 0 (Potassium Channels)
RN  - 0 (Potassium Channels, Calcium-Activated)
RN  - 0 (RNA, Messenger)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
CIN - Science. 1999 Sep 17;285(5435):1859-60. PMID: 10515790
MH  - Animals
MH  - Cattle
MH  - Cell Line
MH  - Electrophysiology
MH  - Estradiol/genetics/*metabolism
MH  - Humans
MH  - Large-Conductance Calcium-Activated Potassium Channel alpha Subunits
MH  - Large-Conductance Calcium-Activated Potassium Channel beta Subunits
MH  - Large-Conductance Calcium-Activated Potassium Channels
MH  - Patch-Clamp Techniques
MH  - Potassium Channels/*metabolism
MH  - *Potassium Channels, Calcium-Activated
MH  - Protein Binding
MH  - RNA, Messenger
MH  - Rats
MH  - Xenopus laevis
EDAT- 1999/09/18 00:00
MHDA- 1999/09/18 00:01
CRDT- 1999/09/18 00:00
PHST- 1999/09/18 00:00 [pubmed]
PHST- 1999/09/18 00:01 [medline]
PHST- 1999/09/18 00:00 [entrez]
AID - 7831 [pii]
AID - 10.1126/science.285.5435.1929 [doi]
PST - ppublish
SO  - Science. 1999 Sep 17;285(5435):1929-31. doi: 10.1126/science.285.5435.1929.