PMID- 10489353
OWN - NLM
STAT- MEDLINE
DCOM- 19991108
LR  - 20041117
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 262
IP  - 1
DP  - 1999 Sep 15
TI  - Analysis of the RNA binding specificity of the human tap protein, a constitutive 
      transport element-specific nuclear RNA export factor.
PG  - 200-9
AB  - The human Tap protein has been proposed to mediate Mason Pfizer monkey virus
      constitutive transport element (CTE)-dependent nuclear RNA export and may also
      play a role in global mRNA export. Here, we have used in vivo assays, in both
      yeast and human cells, together with in vitro assays, to further characterize the
      RNA binding properties of Tap, which has been proposed to contain a novel
      leucine-rich RNA binding motif. Using the yeast three hybrid assay, we selected
      RNA molecules that retain Tap binding activity from a pool of randomized CTE
      sequences. The recovered RNA sequences differed only minimally from the wild-type
      CTE yet all displayed lower affinity for Tap both in vivo and in vitro. Analysis 
      of the RNA export activity of the recovered CTE variants revealed that Tap
      affinity was highly predictive of CTE biological activity. Together, these
      observations provide additional evidence supporting the identification of Tap as 
      the direct cofactor for CTE function and demonstrate that RNA binding by Tap is
      highly sequence specific.
CI  - Copyright 1999 Academic Press.
FAU - Kang, Y
AU  - Kang Y
AD  - Department of Genetics, Duke University Medical Center, Durham, North Carolina
      27710, USA.
FAU - Bogerd, H P
AU  - Bogerd HP
FAU - Yang, J
AU  - Yang J
FAU - Cullen, B R
AU  - Cullen BR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (MEX67 protein, S cerevisiae)
RN  - 0 (NXF1 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nucleocytoplasmic Transport Proteins)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Biological Transport/genetics
MH  - Cell Nucleus/genetics/*metabolism
MH  - Cells, Cultured
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Nucleic Acid Conformation
MH  - *Nucleocytoplasmic Transport Proteins
MH  - Quail
MH  - RNA-Binding Proteins/genetics/*metabolism
MH  - Saccharomyces cerevisiae/genetics/metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Tumor Cells, Cultured
EDAT- 1999/09/18 00:00
MHDA- 1999/09/18 00:01
CRDT- 1999/09/18 00:00
PHST- 1999/09/18 00:00 [pubmed]
PHST- 1999/09/18 00:01 [medline]
PHST- 1999/09/18 00:00 [entrez]
AID - 10.1006/viro.1999.9906 [doi]
AID - S0042-6822(99)99906-3 [pii]
PST - ppublish
SO  - Virology. 1999 Sep 15;262(1):200-9. doi: 10.1006/viro.1999.9906.