PMID- 10488744
OWN - NLM
STAT- MEDLINE
DCOM- 20000131
LR  - 20190826
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 54
IP  - 2
DP  - 1999 Aug
TI  - There is more to HLA-C than exons 2 and 3: sequencing exons 1, 4 and 5.
PG  - 169-77
AB  - HLA-C was shown to be a highly polymorphic gene which can be accurately typed for
      by sequencing methodologies. Most HLA-C sequence-based typing protocols described
      so far are based on analysis of sequence data of exons 2 and 3. Nonetheless,
      exons 1, 4 and 5 also contain nucleotide substitutions which contribute to the
      polymorphisms of the HLA-C locus. Ten alleles contain polymorphic positions in
      exons 1, 4 and 5, Cw*0701/06, Cw*1202112, Cw*15051/2, Cw*1701/02, and Cw*1801/02.
      Here we describe a reliable solid-phase sequence-based typing strategy for
      sequencing exons 1, 4 and 5, which is an extended protocol of our previous HLA-C 
      study. A panel of 16 individuals, carrying 27 different Cw-alleles, was typed for
      exons 1, 4 and 5 to check the newly designed primers. No allelic dropout or
      preferential amplification was noticed in these individuals. The panel was also
      sequenced in order to check the known polymorphisms present in exons 1, 4 and 5. 
      For exon 5 the sequences of the alleles Cw*0302, *0501 and *07011 did not
      correspond with the published data. In addition, exons 1, 4 and 5 were
      sequence-based typing typed in 28, 17 and 59 individuals, respectively. Two new
      alleles were detected which contain polymorphic positions outside exons 2 and 3, 
      Cw*07012 and Cw*1703. The unknown sequence data of exons 1, 4 and 5 of the
      alleles Cw*02024, *0308, *1506 and *16041 were elucidated. The described
      high-resolution sequence-based typing protocol for sequencing exons 1, 4 and 5
      will be a valuable tool to study the HLA-C locus for polymorphisms outside exons 
      2 and 3 and for identification of the presently known HLA-C alleles with
      polymorphic positions in these exons.
FAU - van der Vlies, S A
AU  - van der Vlies SA
AD  - Tissue Typing Laboratory, University hospital Maastricht, The Netherlands.
FAU - Voorter, C E
AU  - Voorter CE
FAU - van den Berg-Loonen, E M
AU  - van den Berg-Loonen EM
LA  - eng
SI  - GENBANK/AJ011881
SI  - GENBANK/AJ011882
SI  - GENBANK/AJ011883
SI  - GENBANK/AJ011884
SI  - GENBANK/Y18139
SI  - GENBANK/Y18140
SI  - GENBANK/Y18141
SI  - GENBANK/Y18142
SI  - GENBANK/Y18143
SI  - GENBANK/Y18144
SI  - GENBANK/Y18145
SI  - GENBANK/Y18146
SI  - GENBANK/Y18499
SI  - GENBANK/Y18533
SI  - GENBANK/Y18534
SI  - GENBANK/Y18535
SI  - GENBANK/Y18536
SI  - GENBANK/Y18537
SI  - GENBANK/Y18538
SI  - GENBANK/Y18539
SI  - GENBANK/Y18540
SI  - GENBANK/Y18541
SI  - GENBANK/Y18656
SI  - GENBANK/Y18659
PT  - Journal Article
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (HLA-C Antigens)
SB  - IM
MH  - Base Sequence
MH  - Cell Line
MH  - Exons/*genetics
MH  - HLA-C Antigens/*genetics
MH  - Humans
MH  - Molecular Sequence Data
EDAT- 1999/09/17 00:00
MHDA- 1999/09/17 00:01
CRDT- 1999/09/17 00:00
PHST- 1999/09/17 00:00 [pubmed]
PHST- 1999/09/17 00:01 [medline]
PHST- 1999/09/17 00:00 [entrez]
AID - 10.1034/j.1399-0039.1999.540208.x [doi]
PST - ppublish
SO  - Tissue Antigens. 1999 Aug;54(2):169-77. doi: 10.1034/j.1399-0039.1999.540208.x.