PMID- 10488337
OWN - NLM
STAT- MEDLINE
DCOM- 19991012
LR  - 20190915
IS  - 1097-2765 (Print)
IS  - 1097-2765 (Linking)
VI  - 4
IP  - 2
DP  - 1999 Aug
TI  - A human nuclear-localized chaperone that regulates dimerization, DNA binding, and
      transcriptional activity of bZIP proteins.
PG  - 219-28
AB  - We have identified and cloned a human nuclear protein that dramatically increases
      DNA binding of transcription factors that contain a basic region-leucine zipper
      (bZIP) DNA binding domain. We show that this bZIP enhancing factor (BEF)
      functions as a molecular chaperone. BEF stimulates DNA binding by recognizing the
      unfolded leucine zipper and promoting the folding of bZIP monomers to dimers; the
      elevated concentration of the bZIP dimer then drives the DNA binding reaction.
      Antisense experiments indicate that BEF is required for efficient transcriptional
      activation by bZIP proteins in vivo. Our results reveal protein folding in the
      nucleus as a step at which sequence-specific DNA binding proteins can be
      regulated.
FAU - Virbasius, C M
AU  - Virbasius CM
AD  - Howard Hughes Medical Institute, Program in Molecular Medicine, University of
      Massachusetts Medical Center, Worcester 01605, USA.
FAU - Wagner, S
AU  - Wagner S
FAU - Green, M R
AU  - Green MR
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell
JT  - Molecular cell
JID - 9802571
RN  - 0 (ALYREF protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Molecular Chaperones)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 2.8.1.1 (Thiosulfate Sulfurtransferase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cell Line
MH  - Cell Nucleus/*metabolism
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/chemistry/genetics/*metabolism
MH  - Dimerization
MH  - *Gene Expression Regulation
MH  - HeLa Cells
MH  - Humans
MH  - Leucine Zippers
MH  - Molecular Chaperones/*metabolism
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/chemistry/*metabolism
MH  - Protein Folding
MH  - *RNA-Binding Proteins
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Thiosulfate Sulfurtransferase/metabolism
MH  - Transcription Factors/chemistry/genetics/*metabolism
MH  - *Transcription, Genetic
MH  - Transcriptional Activation
MH  - Transfection
EDAT- 1999/09/17 00:00
MHDA- 1999/09/17 00:01
CRDT- 1999/09/17 00:00
PHST- 1999/09/17 00:00 [pubmed]
PHST- 1999/09/17 00:01 [medline]
PHST- 1999/09/17 00:00 [entrez]
AID - S1097-2765(00)80369-X [pii]
AID - 10.1016/s1097-2765(00)80369-x [doi]
PST - ppublish
SO  - Mol Cell. 1999 Aug;4(2):219-28. doi: 10.1016/s1097-2765(00)80369-x.