PMID- 10488147
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 39
DP  - 1999 Sep 24
TI  - Molecular cloning of a novel human CC chemokine (Eotaxin-3) that is a functional 
      ligand of CC chemokine receptor 3.
PG  - 27975-80
AB  - Previously, we mapped the novel CC chemokine myeloid progenitor inhibitory factor
      2 (MPIF-2)/eotaxin-2 to chromosome 7q11.23 (Nomiyama, H., Osborne, L. R., Imai,
      T., Kusuda, J., Miura, R., Tsui, L.-C., and Yoshie, O. (1998) Genomics 49,
      339-340). Since chemokine genes tend to be clustered, unknown chemokines may be
      present in the vicinity of those mapped to new chromosomal loci. Prompted by this
      hypothesis, we analyzed the genomic region containing the gene for
      MPIF-2/eotaxin-2 (SCYA24) and have identified a novel CC chemokine termed
      eotaxin-3. The genes for MPIF-2/eotaxin-2 (SCYA24) and eotaxin-3 (SCYA26) are
      localized within a region of approximately 40 kilobases. By Northern blot
      analysis, eotaxin-3 mRNA was constitutively expressed in the heart and ovary. We 
      have generated recombinant eotaxin-3 in a baculovirus expression system.
      Eotaxin-3 induced transient calcium mobilization specifically in CC chemokine
      receptor 3 (CCR3)-expressing L1.2 cells with an EC(50) of 3 nM. Eotaxin-3
      competed the binding of (125)I-eotaxin to CCR3-expressing L1.2 cells with an
      IC(50) of 13 nM. Eotaxin-3 was chemotactic for normal peripheral blood
      eosinophils and basophils at high concentrations. Collectively, eotaxin-3 is yet 
      another functional ligand for CCR3. The potency of eotaxin-3 as a CCR3 ligand
      seems, however, to be approximately 10-fold less than that of eotaxin.
      Identification of eotaxin-3 will further promote our understanding of the control
      of eosinophil trafficking and other CCR3-mediated biological phenomena. The
      strategy used in this study may also be applicable to identification of other
      unknown chemokine genes.
FAU - Kitaura, M
AU  - Kitaura M
AD  - Shionogi Institute for Medical Science, Osaka 566-0022, Kinki University School
      of Medicine, Osaka 589-8511, Japan.
FAU - Suzuki, N
AU  - Suzuki N
FAU - Imai, T
AU  - Imai T
FAU - Takagi, S
AU  - Takagi S
FAU - Suzuki, R
AU  - Suzuki R
FAU - Nakajima, T
AU  - Nakajima T
FAU - Hirai, K
AU  - Hirai K
FAU - Nomiyama, H
AU  - Nomiyama H
FAU - Yoshie, O
AU  - Yoshie O
LA  - eng
SI  - GENBANK/AB010447
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (CCL24 protein, human)
RN  - 0 (CCL26 protein, human)
RN  - 0 (CCR3 protein, human)
RN  - 0 (Ccl24 protein, mouse)
RN  - 0 (Ccr3 protein, mouse)
RN  - 0 (Chemokine CCL24)
RN  - 0 (Chemokine CCL26)
RN  - 0 (Chemokines, CC)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Receptors, CCR3)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Chemokine CCL24
MH  - Chemokine CCL26
MH  - Chemokines, CC/*genetics/*metabolism
MH  - Chemotactic Factors/genetics/metabolism
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 7
MH  - Cloning, Molecular
MH  - Evolution, Molecular
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Multigene Family
MH  - Phylogeny
MH  - Receptors, CCR3
MH  - Receptors, Chemokine/*metabolism
MH  - Recombinant Proteins/metabolism
MH  - Restriction Mapping
MH  - Transfection
EDAT- 1999/09/17 00:00
MHDA- 1999/09/17 00:01
CRDT- 1999/09/17 00:00
PHST- 1999/09/17 00:00 [pubmed]
PHST- 1999/09/17 00:01 [medline]
PHST- 1999/09/17 00:00 [entrez]
AID - 10.1074/jbc.274.39.27975 [doi]
AID - S0021-9258(19)52210-4 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Sep 24;274(39):27975-80. doi: 10.1074/jbc.274.39.27975.