PMID- 10488139
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 39
DP  - 1999 Sep 24
TI  - Y receptor-mediated induction of CD63 transcripts, a tetraspanin determined to be
      necessary for differentiation of the intestinal epithelial cell line, hBRIE 380i 
      cells.
PG  - 27914-24
AB  - Peptide YY (PYY) and neuropeptide Y (NPY) are peptides that coordinate intestinal
      activities in response to luminal and neuronal signals. In this study, using the 
      rat hybrid small intestinal epithelial cell line, hBRIE 380i cells, we
      demonstrated that PYY- and NPY-induced rearrangement of actin filaments may be in
      part through a Y1alpha and/or a nonneuronal Y2 receptor, which were cloned from
      both the intestinal mucosa and the hBRIE 380i cells. A number of
      PYY/NPY-responsive genes were also identified by subtractive hybridization of the
      hBRIE 380i cells in the presence or absence of a 6-h treatment with PYY. Several 
      of these genes coded for proteins associated with the cell cytoskeleton or
      extracellular matrix. One of these proteins was the transmembrane-4 superfamily
      protein CD63, previously shown to associate with beta(1)-integrin and implicated 
      in cell adhesion. CD63 immunoreactivity, using antibody to the extracellular
      domain, was highest in the differentiated cell clusters of the hBRIE 380i cells. 
      The hBRIE 380i cells transfected with antisense CD63 cDNA lost these
      differentiated clusters. These studies suggest a new role for NPY and PYY in
      modulating differentiation through cytoskeletal associated proteins.
FAU - Hallden, G
AU  - Hallden G
AD  - Department of Nutritional Sciences, University of California, Berkeley,
      California 94720-3104, USA.
FAU - Hadi, M
AU  - Hadi M
FAU - Hong, H T
AU  - Hong HT
FAU - Aponte, G W
AU  - Aponte GW
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Antigens, CD)
RN  - 0 (Cd63 protein, rat)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Integrin beta1)
RN  - 0 (Neuropeptide Y)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Gastrointestinal Hormone)
RN  - 0 (Receptors, Neuropeptide Y)
RN  - 0 (Tetraspanin 30)
RN  - 0 (peptide YY receptor)
RN  - 106388-42-5 (Peptide YY)
SB  - IM
MH  - Animals
MH  - Antigens, CD/*genetics/*physiology
MH  - Cell Adhesion Molecules/genetics/physiology
MH  - Cell Differentiation
MH  - Cell Line
MH  - Cytoskeletal Proteins/genetics
MH  - Cytoskeleton/drug effects/physiology/ultrastructure
MH  - Extracellular Matrix Proteins/genetics
MH  - Gene Expression Regulation/drug effects
MH  - Hybrid Cells
MH  - Integrin beta1/genetics/physiology
MH  - Intestinal Mucosa
MH  - Intestine, Small
MH  - Kinetics
MH  - Neuropeptide Y/*pharmacology
MH  - Peptide YY/*pharmacology
MH  - Platelet Membrane Glycoproteins/*genetics/*physiology
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Receptors, Gastrointestinal Hormone/*physiology
MH  - Receptors, Neuropeptide Y/*physiology
MH  - Tetraspanin 30
MH  - *Transcription, Genetic/drug effects
EDAT- 1999/09/17 00:00
MHDA- 1999/09/17 00:01
CRDT- 1999/09/17 00:00
PHST- 1999/09/17 00:00 [pubmed]
PHST- 1999/09/17 00:01 [medline]
PHST- 1999/09/17 00:00 [entrez]
AID - 10.1074/jbc.274.39.27914 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Sep 24;274(39):27914-24. doi: 10.1074/jbc.274.39.27914.