PMID- 10488131
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 39
DP  - 1999 Sep 24
TI  - The mammalian HSF4 gene generates both an activator and a repressor of heat shock
      genes by alternative splicing.
PG  - 27845-56
AB  - The expression of heat shock genes is controlled at the level of transcription by
      members of the heat shock transcription factor family in vertebrates. HSF4 is a
      mammalian factor characterized by its lack of a suppression domain that modulates
      formation of DNA-binding homotrimer. Here, we have determined the exon structure 
      of the human HSF4 gene and identified a major new isoform, HSF4b, derived by
      alternative RNA splicing events, in addition to a previously reported HSF4a
      isoform. In mouse tissues HSF4b mRNA was more abundant than HSF4a as examined by 
      reverse transcription-polymerase chain reaction, and its protein was detected in 
      the brain and lung. Although both mouse HSF4a and HSF4b form trimers in the
      absence of stress, these two isoforms exhibit different transcriptional activity;
      HSF4a acts as an inhibitor of the constitutive expression of heat shock genes,
      and hHSF4b acts as a transcriptional activator. Furthermore HSF4b but not HSF4a
      complements the viability defect of yeast cells lacking HSF. Moreover, heat shock
      and other stresses stimulate transcription of target genes by HSF4b in both yeast
      and mammalian cells. These results suggest that differential splicing of HSF4
      mRNA gives rise to both an inhibitor and activator of tissue-specific heat shock 
      gene expression.
FAU - Tanabe, M
AU  - Tanabe M
AD  - Department of Molecular and Cell Biology, Institute for Frontier Medical
      Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8397, Japan.
FAU - Sasai, N
AU  - Sasai N
FAU - Nagata, K
AU  - Nagata K
FAU - Liu, X D
AU  - Liu XD
FAU - Liu, P C
AU  - Liu PC
FAU - Thiele, D J
AU  - Thiele DJ
FAU - Nakai, A
AU  - Nakai A
LA  - eng
SI  - GENBANK/AB029347
SI  - GENBANK/AB029348
SI  - GENBANK/AB029349
SI  - GENBANK/AB029350
GR  - GM18858/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (HSF4 protein, human)
RN  - 0 (Heat Shock Transcription Factors)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Hsf4 protein, mouse)
RN  - 0 (Protein Isoforms)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - *Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Consensus Sequence
MH  - DNA-Binding Proteins/*genetics/*metabolism
MH  - Exons
MH  - *Gene Expression Regulation
MH  - Heat Shock Transcription Factors
MH  - Heat-Shock Proteins/*genetics
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Protein Isoforms/genetics/metabolism
MH  - Recombinant Proteins/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Saccharomyces cerevisiae/genetics/growth & development
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Transcription Factors/*genetics/*metabolism
MH  - *Transcription, Genetic
MH  - Transfection
MH  - Vertebrates
EDAT- 1999/09/17 00:00
MHDA- 1999/09/17 00:01
CRDT- 1999/09/17 00:00
PHST- 1999/09/17 00:00 [pubmed]
PHST- 1999/09/17 00:01 [medline]
PHST- 1999/09/17 00:00 [entrez]
AID - 10.1074/jbc.274.39.27845 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Sep 24;274(39):27845-56. doi: 10.1074/jbc.274.39.27845.