PMID- 10488122
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 39
DP  - 1999 Sep 24
TI  - Molecular cloning and characterization of a novel human G-protein-coupled
      receptor, EDG7, for lysophosphatidic acid.
PG  - 27776-85
AB  - Lysophosphatidic acid (LPA), together with sphingosine 1-phosphate, is a
      bioactive lipid mediator that acts on G-protein-coupled receptors to evoke
      multiple cellular responses, including Ca(2+) mobilization, modulation of
      adenylyl cyclase, and mitogen-activated protein (MAP) kinase activation. In this 
      study, we isolated a human cDNA encoding a novel G-protein-coupled receptor,
      designated EDG7, and characterized it as a cellular receptor for LPA. The amino
      acid sequence of the EDG7 protein is 53.7 and 48.8% identical to those of the
      human functional LPA receptors EDG2 and EDG4, respectively, previously
      identified. LPA (oleoyl) but not other lysophospholipids induced an increase in
      the [Ca(2+)](i) of EDG7-overexpressing Sf9 cells. Other LPA receptors, EDG4 but
      not EDG2, transduced the Ca(2+) response by LPA when expressed in Sf9 cells. LPAs
      with an unsaturated fatty acid but not with a saturated fatty acid induced an
      increase in the [Ca(2+)](i) of EDG7-expressing Sf9 cells, whereas LPAs with both 
      saturated and unsaturated fatty acids elicited a Ca(2+) response in Sf9 cells
      expressing EDG4. In EDG7- or EDG4-expressing Sf9 cells, LPA stimulated
      forskolin-induced increase in intracellular cAMP levels, which was not observed
      in EDG2-expressing cells. In PC12 cells, EDG4 but not EDG2 or EDG7 mediated the
      activation of MAP kinase by LPA. Neither the EDG7- nor EDG4-transduced Ca(2+)
      response or cAMP accumulation was inhibited by pertussis toxin. In conclusion,
      the present study demonstrates that EDG7, a new member of the EDG family of
      G-protein-coupled receptors, is a specific LPA receptor that shows distinct
      properties from known cloned LPA receptors in ligand specificities, Ca(2+)
      response, modulation of adenylyl cyclase, and MAP kinase activation.
FAU - Bandoh, K
AU  - Bandoh K
AD  - Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo,
      Bunkyo-ku, Tokyo 113-0033, Japan.
FAU - Aoki, J
AU  - Aoki J
FAU - Hosono, H
AU  - Hosono H
FAU - Kobayashi, S
AU  - Kobayashi S
FAU - Kobayashi, T
AU  - Kobayashi T
FAU - Murakami-Murofushi, K
AU  - Murakami-Murofushi K
FAU - Tsujimoto, M
AU  - Tsujimoto M
FAU - Arai, H
AU  - Arai H
FAU - Inoue, K
AU  - Inoue K
LA  - eng
SI  - GENBANK/AF127138
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA Primers)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Elk1 protein, rat)
RN  - 0 (Lysophospholipids)
RN  - 0 (Potassium Channels)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Receptors, Lysophosphatidic Acid)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (ets-Domain Protein Elk-1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Calcium/metabolism
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - *DNA-Binding Proteins
MH  - Female
MH  - GTP-Binding Proteins/*metabolism
MH  - Humans
MH  - Kinetics
MH  - Lysophospholipids/*metabolism
MH  - Male
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - PC12 Cells
MH  - Phylogeny
MH  - Potassium Channels/metabolism
MH  - *Proto-Oncogene Proteins
MH  - Rats
MH  - Receptors, Cell Surface/chemistry/genetics/*metabolism
MH  - *Receptors, G-Protein-Coupled
MH  - Receptors, Lysophosphatidic Acid
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Spodoptera
MH  - *Transcription Factors
MH  - Transfection
MH  - ets-Domain Protein Elk-1
EDAT- 1999/09/17 00:00
MHDA- 1999/09/17 00:01
CRDT- 1999/09/17 00:00
PHST- 1999/09/17 00:00 [pubmed]
PHST- 1999/09/17 00:01 [medline]
PHST- 1999/09/17 00:00 [entrez]
AID - 10.1074/jbc.274.39.27776 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Sep 24;274(39):27776-85. doi: 10.1074/jbc.274.39.27776.