PMID- 10488094
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 39
DP  - 1999 Sep 24
TI  - A relationship between protein kinase C phosphorylation and calmodulin binding to
      the metabotropic glutamate receptor subtype 7.
PG  - 27573-7
AB  - Metabotropic glutamate receptor subtype 7 (mGluR7) is coupled to the inhibitory
      cyclic AMP cascade and is selectively activated by a glutamate analogue,
      L-2-amino-4-phosphonobutyrate. Among L-2-amino-4-phosphonobutyrate-sensitive
      mGluR subtypes, mGluR7 is highly concentrated at the presynaptic terminals and is
      thought to play an important role in modulation of glutamatergic synaptic
      transmission by presynaptic inhibition of glutamate release. To gain further
      insight into the intracellular signaling mechanisms of mGluR7, with the aid of
      glutathione S-transferase fusion affinity chromatography, we attempted to
      identify proteins that interact with the intracellular carboxyl terminus of
      mGluR7. Here, we report that calmodulin (CaM) directly binds to the carboxyl
      terminus of mGluR7 in a Ca(2+)-dependent manner. The CaM-binding domain is
      located immediately following the 7th transmembrane segment. We also show that
      the CaM-binding domain of mGluR7 is phosphorylated by protein kinase C (PKC).
      This phosphorylation is inhibited by the binding of Ca(2+)/CaM to the receptor.
      Conversely, the Ca(2+)/CaM binding is prevented by PKC phosphorylation.
      Collectively, these results suggest that mGluR7 serves to cross-link the cyclic
      AMP, Ca(2+), and PKC phosphorylation signal transduction cascades.
FAU - Nakajima, Y
AU  - Nakajima Y
AD  - Department of Cell Physiology, National Institute for Physiological Sciences,
      Okazaki 444-8585, Japan.
FAU - Yamamoto, T
AU  - Yamamoto T
FAU - Nakayama, T
AU  - Nakayama T
FAU - Nakanishi, S
AU  - Nakanishi S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (2-amino-4-phosphono-propinate)
RN  - 0 (Calmodulin)
RN  - 0 (Propionates)
RN  - 0 (Receptor, Metabotropic Glutamate 5)
RN  - 0 (Receptors, Metabotropic Glutamate)
RN  - 0 (Recombinant Proteins)
RN  - 0 (metabotropic glutamate receptor 7)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - YZN9W7P1BX (metabotropic glutamate receptor 4)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amino Acid Substitution
MH  - Animals
MH  - Binding Sites
MH  - Brain/*metabolism
MH  - Calmodulin/*metabolism
MH  - Chromatography, Affinity
MH  - Kinetics
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Phosphorylation
MH  - Presynaptic Terminals/metabolism
MH  - Propionates/pharmacology
MH  - Protein Kinase C/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, Metabotropic Glutamate 5
MH  - Receptors, Metabotropic Glutamate/chemistry/isolation & purification/*metabolism
MH  - Recombinant Proteins/chemistry/isolation & purification/metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
EDAT- 1999/09/17 00:00
MHDA- 1999/09/17 00:01
CRDT- 1999/09/17 00:00
PHST- 1999/09/17 00:00 [pubmed]
PHST- 1999/09/17 00:01 [medline]
PHST- 1999/09/17 00:00 [entrez]
AID - 10.1074/jbc.274.39.27573 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Sep 24;274(39):27573-7. doi: 10.1074/jbc.274.39.27573.