PMID- 10488079
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 39
DP  - 1999 Sep 24
TI  - Synamon, a novel neuronal protein interacting with synapse-associated protein
      90/postsynaptic density-95-associated protein.
PG  - 27463-6
AB  - Guanylate kinase-associated protein (GKAP)/SAP90/PSD-95-associated protein
      (SAPAP)/DLG-associated protein (DAP) is a protein of the postsynaptic density
      (PSD), and binds to the guanylate kinase domain of PSD-95/synapse-associated
      protein (SAP) 90 and synaptic scaffolding molecule. GKAP/SAPAP/DAP recruits
      PSD-95/SAP90 and its interacting protein, brain-enriched guanylate
      kinase-interacting protein, into the Triton X-100-insoluble fraction in
      transfected cells, suggesting that GKAP/SAPAP/DAP may link several PSD components
      to the Triton X-100-insoluble structures in the PSD. We have identified here a
      novel neuronal GKAP/SAPAP/DAP-binding protein and named it synamon. Synamon has
      seven ankyrin repeats at the NH(2) terminus followed by one src homology 3 domain
      and one PSD-95/Dlg-A/ZO-1 domain, and several proline-rich regions at the
      carboxyl terminus. Synamon interacts with the COOH-terminal region of
      GKAP/SAPAP/DAP via the middle region containing a PSD-95/Dlg-A/ZO-1 domain.
      Synamon was coimmunoprecipitated with SAPAP from rat crude synaptosomes and
      colocalized with SAPAP in primary cultured rat hippocampal neurons. Because
      synamon is composed of various protein-interacting modules, it may also interact 
      with proteins other than GKAP/SAPAP/DAP to organize the architecture of the PSD.
FAU - Yao, I
AU  - Yao I
AD  - Takai Biotimer Project, Exploratory Research for Advanced Technology, Japan
      Science and Technology Corporation, c/o JCR Pharmaceuticals Co. Ltd., 2-2-10
      Murotani, Nishi-ku, Kobe 651-2241, Japan.
FAU - Hata, Y
AU  - Hata Y
FAU - Hirao, K
AU  - Hirao K
FAU - Deguchi, M
AU  - Deguchi M
FAU - Ide, N
AU  - Ide N
FAU - Takeuchi, M
AU  - Takeuchi M
FAU - Takai, Y
AU  - Takai Y
LA  - eng
SI  - GENBANK/AF102855
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (Disks Large Homolog 4 Protein)
RN  - 0 (Dlg4 protein, rat)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (SAP90-PSD95 Associated Proteins)
RN  - 0 (Shank1 protein, rat)
RN  - 0 (postsynaptic density proteins)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brain/*metabolism
MH  - Carrier Proteins/*chemistry/genetics/*metabolism
MH  - Cloning, Molecular
MH  - Disks Large Homolog 4 Protein
MH  - Gene Library
MH  - Hippocampus/metabolism
MH  - Intracellular Signaling Peptides and Proteins
MH  - Membrane Proteins
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/*chemistry/genetics/*metabolism
MH  - Neurons/*metabolism
MH  - Rats
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Repetitive Sequences, Amino Acid
MH  - SAP90-PSD95 Associated Proteins
MH  - Transfection
MH  - src Homology Domains
EDAT- 1999/09/17 00:00
MHDA- 1999/09/17 00:01
CRDT- 1999/09/17 00:00
PHST- 1999/09/17 00:00 [pubmed]
PHST- 1999/09/17 00:01 [medline]
PHST- 1999/09/17 00:00 [entrez]
AID - 10.1074/jbc.274.39.27463 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Sep 24;274(39):27463-6. doi: 10.1074/jbc.274.39.27463.