PMID- 10488076
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 39
DP  - 1999 Sep 24
TI  - Transforming growth factor-beta-stimulated clone-22 is a member of a family of
      leucine zipper proteins that can homo- and heterodimerize and has transcriptional
      repressor activity.
PG  - 27439-47
AB  - TGF-beta-stimulated clone-22 (TSC-22) encodes a leucine zipper-containing protein
      that is highly conserved during evolution. Two homologues are known that share a 
      similar leucine zipper domain and another conserved domain (designated the TSC
      box). Only limited data are available on the function of TSC-22 and its
      homologues. TSC-22 is transcriptionally up-regulated by many different stimuli,
      including anti-cancer drugs and growth inhibitors, and recent data suggest that
      TSC-22 may play a suppressive role in tumorigenesis. In this paper we show that
      TSC-22 forms homodimers via its conserved leucine zipper domain. Using a yeast
      two-hybrid screen, we identified a TSC-22 homologue (THG-1) as heterodimeric
      partner. Furthermore, we report the presence of two more mammalian family members
      with highly conserved leucine zippers and TSC boxes. Interestingly, both TSC-22
      and THG-1 have transcriptional repressor activity when fused to a heterologous
      DNA-binding domain. The repressor activity of TSC-22 appears sensitive for
      promoter architecture, but not for the histone deacetylase inhibitor trichostatin
      A. Mutational analysis showed that this repressor activity resides in the
      non-conserved regions of the protein and is enhanced by the conserved
      dimerization domain. Our results suggest that TSC-22 belongs to a family of
      leucine zipper-containing transcription factors that can homodimerize and
      heterodimerize with other family members and that at least two TSC-22 family
      members may be repressors of transcription.
FAU - Kester, H A
AU  - Kester HA
AD  - Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan
      8, 3584 CT Utrecht, The Netherlands.
FAU - Blanchetot, C
AU  - Blanchetot C
FAU - den Hertog, J
AU  - den Hertog J
FAU - van der Saag, P T
AU  - van der Saag PT
FAU - van der Burg, B
AU  - van der Burg B
LA  - eng
SI  - GENBANK/AJ133115
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (TSC22D1 protein, human)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - COS Cells
MH  - Conserved Sequence
MH  - Dimerization
MH  - Evolution, Molecular
MH  - *Gene Expression Regulation
MH  - Humans
MH  - *Leucine Zippers
MH  - Molecular Sequence Data
MH  - Recombinant Fusion Proteins/metabolism
MH  - Repressor Proteins/chemistry/*genetics/*metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - *Transcription, Genetic
MH  - Transfection
EDAT- 1999/09/17 00:00
MHDA- 1999/09/17 00:01
CRDT- 1999/09/17 00:00
PHST- 1999/09/17 00:00 [pubmed]
PHST- 1999/09/17 00:01 [medline]
PHST- 1999/09/17 00:00 [entrez]
AID - 10.1074/jbc.274.39.27439 [doi]
AID - S0021-9258(19)52139-1 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Sep 24;274(39):27439-47. doi: 10.1074/jbc.274.39.27439.