PMID- 10488071
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 39
DP  - 1999 Sep 24
TI  - JAZ requires the double-stranded RNA-binding zinc finger motifs for nuclear
      localization.
PG  - 27399-406
AB  - We have cloned and characterized a novel zinc finger protein, termed JAZ. JAZ
      contains four C(2)H(2)-type zinc finger motifs that are connected by long (28-38)
      amino acid linker sequences. JAZ is expressed in all tissues tested and localizes
      in the nucleus, primarily the nucleolus. JAZ preferentially binds to
      double-stranded (ds) RNA or RNA/DNA hybrids rather than DNA. Mutation of
      individual zinc finger motifs reveals that the zinc finger domains are not only
      essential for dsRNA binding but are also required for its nucleolar localization,
      which demonstrates a complex trafficking mechanism dependent on the nucleic
      acid-binding capability of the protein. Furthermore, forced expression of JAZ
      potently induces apoptosis in murine fibroblast cells. Thus, JAZ may belong to a 
      class of zinc finger proteins that features dsRNA binding and may regulate cell
      growth via the unique dsRNA binding properties.
FAU - Yang, M
AU  - Yang M
AD  - Sealy Center for Oncology and Hematology, Department of Internal Medicine, The
      University of Texas Medical Branch, Galveston, Texas 77555-1048, USA.
FAU - May, W S
AU  - May WS
FAU - Ito, T
AU  - Ito T
LA  - eng
SI  - GENBANK/AF083339
SI  - GENBANK/AF083340
GR  - CA44649/CA/NCI NIH HHS/United States
GR  - HL54083/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (RNA, Double-Stranded)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (ZNF346 protein, human)
RN  - 0 (Zfp346 protein, mouse)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Apoptosis
MH  - COS Cells
MH  - Cell Nucleus/metabolism
MH  - DNA/metabolism
MH  - DNA-Binding Proteins/*chemistry/genetics/*metabolism
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Nucleic Acid Hybridization
MH  - Polymerase Chain Reaction
MH  - RNA/metabolism
MH  - RNA, Double-Stranded/*metabolism
MH  - RNA-Binding Proteins/*chemistry/genetics/*metabolism
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Transfection
MH  - *Zinc Fingers
EDAT- 1999/09/17 00:00
MHDA- 1999/09/17 00:01
CRDT- 1999/09/17 00:00
PHST- 1999/09/17 00:00 [pubmed]
PHST- 1999/09/17 00:01 [medline]
PHST- 1999/09/17 00:00 [entrez]
AID - 10.1074/jbc.274.39.27399 [doi]
AID - S0021-9258(19)52134-2 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Sep 24;274(39):27399-406. doi: 10.1074/jbc.274.39.27399.