PMID- 10488063
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 39
DP  - 1999 Sep 24
TI  - Characterization of a novel kinetochore protein, CENP-H.
PG  - 27343-6
AB  - Macromolecular centromere-kinetochore complex plays a critical role in sister
      chromatid separation, but its complete protein composition as well as its precise
      dynamic function during mitosis has not yet been clearly determined. Here we
      report the isolation of a novel mouse kinetochore protein, CENP-H. The CENP-H,
      with an apparent molecular mass of 33 kDa, was found to contain a coiled-coil
      structure and a nuclear localization signal. The CENP-H transcripts were
      relatively scarce but were detectable in most tissues and embryos at various
      stages of development. Immunofluorescence stainings of mouse fibroblast cells
      with anti-CENP-H-specific antibody demonstrated that the CENP-H is specifically
      and constitutively localized in kinetochores throughout the cell cycle; this was 
      also confirmed by stainings with anti-centromere-specific antibody. Thus the
      newly isolated CENP-H may play a role in kinetochore organization and function
      throughout the cell cycle.
FAU - Sugata, N
AU  - Sugata N
AD  - Tsukuba Life Science Center, The Institute of Physical and Chemical Research
      (RIKEN), 3-1, Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
FAU - Munekata, E
AU  - Munekata E
FAU - Todokoro, K
AU  - Todokoro K
LA  - eng
SI  - GENBANK/AB017634
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (CENPH protein, human)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (Recombinant Proteins)
RN  - 11096-26-7 (Erythropoietin)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chromosomal Proteins, Non-Histone/chemistry/*genetics/*metabolism
MH  - Cloning, Molecular
MH  - Embryonic and Fetal Development
MH  - Erythropoietin/pharmacology
MH  - Female
MH  - *Gene Expression Regulation, Developmental
MH  - Kinetochores/*physiology
MH  - Leukemia, Erythroblastic, Acute
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Recombinant Proteins/biosynthesis/chemistry
MH  - Transcription, Genetic/drug effects
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1999/09/17 00:00
MHDA- 1999/09/17 00:01
CRDT- 1999/09/17 00:00
PHST- 1999/09/17 00:00 [pubmed]
PHST- 1999/09/17 00:01 [medline]
PHST- 1999/09/17 00:00 [entrez]
AID - 10.1074/jbc.274.39.27343 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Sep 24;274(39):27343-6. doi: 10.1074/jbc.274.39.27343.