PMID- 10487848
OWN - NLM
STAT- MEDLINE
DCOM- 19991007
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 0002-9440 (Linking)
VI  - 155
IP  - 3
DP  - 1999 Sep
TI  - Cloning and expression of the rat nephrin homolog.
PG  - 907-13
AB  - Despite of the increased availability of genetically modified mouse strains, the 
      experimental models in the rat have provided the most widely employed and
      versatile models for the study of renal pathophysiology and functional genetics. 
      The identification of the human gene mutated in the congenital nephrotic syndrome
      of the Finnish type (NPHS1) has recently been reported, and its protein product
      has been termed nephrin. Here we report the molecular cloning and
      characterization of rat nephrin cDNA. Rat nephrin cDNA has an open reading frame 
      of 3705 bp, shows 82% sequence identity with human nephrin cDNA, and shows
      characteristic rat-specific splicing variants. The translated nucleotide sequence
      has 89% sequence identity at the amino acid level. The signal sequence,
      glycosylation, and cysteine localization patterns are nearly identical to those
      of human nephrin. As in the human, the rat nephrin transcript is expressed in a
      tissue-restricted pattern. Antipeptide antibodies raised to the intracellular
      nephrin-specific domain identified immunoreactivity exclusively within the rat
      kidney glomerulus by indirect immunofluorescence. Initial results with
      semiquantitative reverse transcriptase-polymerase chain reaction analysis showed 
      a remarkable down-regulation of nephrin-specific mRNA in the puromycin nephrosis 
      of the rat.
FAU - Ahola, H
AU  - Ahola H
AD  - Division of Bacteriology, Haartman Institute, University of Helsinki, Helsinki,
      Finland.
FAU - Wang, S X
AU  - Wang SX
FAU - Luimula, P
AU  - Luimula P
FAU - Solin, M L
AU  - Solin ML
FAU - Holzman, L B
AU  - Holzman LB
FAU - Holthofer, H
AU  - Holthofer H
LA  - eng
SI  - GENBANK/AF125521
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Proteins)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (nephrin)
RN  - 58-60-6 (Puromycin Aminonucleoside)
SB  - AIM
SB  - IM
MH  - Alternative Splicing/genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Northern
MH  - *Cloning, Molecular
MH  - DNA, Complementary/genetics/isolation & purification
MH  - Down-Regulation
MH  - Fluorescent Antibody Technique, Indirect
MH  - Humans
MH  - Kidney Cortex/metabolism
MH  - Male
MH  - Membrane Proteins
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nephrosis/chemically induced/metabolism
MH  - Polymerase Chain Reaction
MH  - *Protein Biosynthesis
MH  - Proteins/*genetics
MH  - Puromycin Aminonucleoside
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution/genetics
PMC - PMC1866902
EDAT- 1999/09/17 00:00
MHDA- 1999/09/17 00:01
CRDT- 1999/09/17 00:00
PHST- 1999/09/17 00:00 [pubmed]
PHST- 1999/09/17 00:01 [medline]
PHST- 1999/09/17 00:00 [entrez]
AID - S0002-9440(10)65190-5 [pii]
AID - 10.1016/S0002-9440(10)65190-5 [doi]
PST - ppublish
SO  - Am J Pathol. 1999 Sep;155(3):907-13. doi: 10.1016/S0002-9440(10)65190-5.