PMID- 10487747
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20200204
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 18
DP  - 1999 Sep 15
TI  - GRASP55, a second mammalian GRASP protein involved in the stacking of Golgi
      cisternae in a cell-free system.
PG  - 4949-60
AB  - We have identified a 55 kDa protein, named GRASP55 (Golgi reassembly stacking
      protein of 55 kDa), as a component of the Golgi stacking machinery. GRASP55 is
      homologous to GRASP65, an N-ethylmaleimide-sensitive membrane protein required
      for the stacking of Golgi cisternae in a cell-free system. GRASP65 exists in a
      complex with the vesicle docking protein receptor GM130 to which it binds
      directly, and the membrane tethering protein p115, which also functions in the
      stacking of Golgi cisternae. GRASP55 binding to GM130, could not be detected
      using biochemical methods, although a weak interaction was detected with the
      yeast two-hybrid system. Cryo-electron microscopy revealed that GRASP65, like
      GM130, is present on the cis-Golgi, while GRASP55 is on the medial-Golgi.
      Recombinant GRASP55 and antibodies to the protein block the stacking of Golgi
      cisternae, which is similar to the observations made for GRASP65. These results
      demonstrate that GRASP55 and GRASP65 function in the stacking of Golgi cisternae.
FAU - Shorter, J
AU  - Shorter J
AD  - Cell Biology Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, 
      London WC2A 3PX.
FAU - Watson, R
AU  - Watson R
FAU - Giannakou, M E
AU  - Giannakou ME
FAU - Clarke, M
AU  - Clarke M
FAU - Warren, G
AU  - Warren G
FAU - Barr, F A
AU  - Barr FA
LA  - eng
SI  - GENBANK/AF110267
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Autoantigens)
RN  - 0 (DNA Primers)
RN  - 0 (GORASP1 protein, human)
RN  - 0 (GORASP2 protein, human)
RN  - 0 (Golgi Matrix Proteins)
RN  - 0 (Golgin subfamily A member 2)
RN  - 0 (Gorasp1 protein, rat)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Membrane Proteins)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Autoantigens
MH  - Base Sequence
MH  - Cell-Free System
MH  - Cloning, Molecular
MH  - Cryoelectron Microscopy
MH  - DNA Primers/genetics
MH  - Golgi Apparatus/*metabolism/ultrastructure
MH  - Golgi Matrix Proteins
MH  - HeLa Cells
MH  - Humans
MH  - In Vitro Techniques
MH  - Macromolecular Substances
MH  - Membrane Proteins/*chemistry/genetics/*metabolism
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - Rats
MH  - Recombinant Proteins/chemistry/genetics/metabolism
MH  - Sequence Homology, Amino Acid
PMC - PMC1171566
EDAT- 1999/09/16 00:00
MHDA- 1999/09/16 00:01
CRDT- 1999/09/16 00:00
PHST- 1999/09/16 00:00 [pubmed]
PHST- 1999/09/16 00:01 [medline]
PHST- 1999/09/16 00:00 [entrez]
AID - 10.1093/emboj/18.18.4949 [doi]
PST - ppublish
SO  - EMBO J. 1999 Sep 15;18(18):4949-60. doi: 10.1093/emboj/18.18.4949.