PMID- 10486281
OWN - NLM
STAT- MEDLINE
DCOM- 19991021
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 263
IP  - 1
DP  - 1999 Sep 16
TI  - ITIH4 serum concentration increases during acute-phase processes in human
      patients and is up-regulated by interleukin-6 in hepatocarcinoma HepG2 cells.
PG  - 224-9
AB  - The serum concentration of the inter-alpha trypsin inhibitor heavy chain 4
      protein (ITIH4) increases (from 1.4-3 times) in male patients suffering of
      different acute-phase processes (myocardial infarction, unstable angina or
      programmed surgery). The concentration of C-reactive protein (CRP) in these
      samples ranged from 15 microg/ml to 133 microg/ml. Using the hepatocarcinoma
      HepG2 cell line we have observed up-regulation of ITIH4 mRNA expression upon
      dose-response treatments with interleukin-6 (IL-6). This effect correlates with
      the increase of radiolabeled ITIH4 in the cellular media of (35)S-labeled HepG2
      cells treated with the cytokine. A similar effect was observed for haptoglobin
      mRNA, used as a control for acute-phase protein expression. IL-1beta, although
      up-regulating the expression of alpha(1)-acid glycoprotein in these cells, did
      not induce any effect in the expression of ITIH4. No changes were observed after 
      TNF-alpha treatments. The results presented here indicate that ITIH4 is a type II
      acute-phase protein in humans.
CI  - Copyright 1999 Academic Press.
FAU - Pineiro, M
AU  - Pineiro M
AD  - Facultad de Ciencias, Universidad de Zaragoza, Zaragoza, E-50009, Spain.
      pineiro@posta.unizar.es
FAU - Alava, M A
AU  - Alava MA
FAU - Gonzalez-Ramon, N
AU  - Gonzalez-Ramon N
FAU - Osada, J
AU  - Osada J
FAU - Lasierra, P
AU  - Lasierra P
FAU - Larrad, L
AU  - Larrad L
FAU - Pineiro, A
AU  - Pineiro A
FAU - Lampreave, F
AU  - Lampreave F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (Glycoproteins)
RN  - 0 (Haptoglobins)
RN  - 0 (Interleukin-6)
RN  - 0 (Itih4 protein, mouse)
RN  - 0 (Orosomucoid)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
SB  - IM
MH  - Acute-Phase Reaction/*blood
MH  - Adult
MH  - Aged
MH  - Angina, Unstable/blood
MH  - Base Sequence
MH  - Calcium-Binding Proteins/biosynthesis/*blood/*genetics
MH  - Carcinoma, Hepatocellular/*genetics
MH  - DNA Primers/genetics
MH  - Glycoproteins/biosynthesis/*blood/*genetics
MH  - Haptoglobins/biosynthesis/genetics
MH  - Humans
MH  - Interleukin-6/*pharmacology
MH  - Liver Neoplasms/*genetics
MH  - Male
MH  - Middle Aged
MH  - Myocardial Infarction/blood
MH  - Orosomucoid/biosynthesis/genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA, Neoplasm/genetics/metabolism
MH  - Tumor Cells, Cultured
MH  - Up-Regulation/drug effects
EDAT- 1999/09/16 00:00
MHDA- 1999/09/16 00:01
CRDT- 1999/09/16 00:00
PHST- 1999/09/16 00:00 [pubmed]
PHST- 1999/09/16 00:01 [medline]
PHST- 1999/09/16 00:00 [entrez]
AID - 10.1006/bbrc.1999.1349 [doi]
AID - S0006291X99913499 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Sep 16;263(1):224-9. doi:
      10.1006/bbrc.1999.1349.